Automated F(C)CS analysis of clone # 0827

Created on 12/08/2014
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Gene and protein information

Clone # 0827
Gene symbol: TARDBP
Description: TAR DNA binding protein
Molecular weight (with FP): 71.7 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
0827_img_ch1.png0827_img_ch2.png


Point 1, intensity traces and correlation functions
0827_itr_1.png0827_cor_1.png

Point 2, intensity traces and correlation functions
0827_itr_2.png0827_cor_2.png

Point 3, intensity traces and correlation functions
0827_itr_3.png0827_cor_3.png

Point 4, intensity traces and correlation functions
0827_itr_4.png0827_cor_4.png

Point 5, intensity traces and correlation functions
0827_itr_5.png0827_cor_5.png

Point 6, intensity traces and correlation functions
0827_itr_6.png0827_cor_6.png

Sessions

1 20110915\U03--V00--0827
2 20110920\U03--V01--0827
3 20110921\U02--V00--0827
4 20110927\U00--V01--0827

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.348E+2 9.322E+1 9.527E+2 4.228E+2 2.301E+1 2.039E+1 2.528E+1 3.346E+1 3.705E+2 2.185E+2 1.271E+1 1.635E+1
N_corr 1.766E+2 1.377E+2 1.291E+3 7.119E+2 3.867E+1 3.768E+1 3.292E+1 5.450E+1 5.194E+2 2.960E+2 1.640E+1 2.060E+1
c [nM] 8.382E+2 5.797E+2 4.419E+3 1.961E+3 1.183E+2 1.048E+2 1.572E+2 2.081E+2 1.719E+3 1.013E+3 6.534E+1 8.405E+1
c_corr [nM] 1.098E+3 8.563E+2 5.988E+3 3.302E+3 1.988E+2 1.937E+2 2.048E+2 3.390E+2 2.409E+3 1.373E+3 8.431E+1 1.059E+2
ratioG 1.262E-1 6.501E-2 1.176E-1 7.297E-2
ratioG_corr 2.264E-2 2.543E-2 8.639E-2 5.540E-2
f_1 7.530E-1 8.860E-2 5.414E-1 2.445E-1 9.535E-1 8.002E-2 8.218E-1 6.859E-2 3.699E-1 3.761E-1 8.703E-1 1.835E-1
tauD_1 [ms] 1.969E+0 7.664E-1 4.686E+0 1.037E+1 1.277E+0 8.586E-1 1.086E+0 4.853E-1 1.800E+0 0.000E+0 2.364E+0 3.887E-1
D_1 [mum^2/s] 6.109E+0 2.055E+0 6.615E+0 1.839E+0 1.924E+1 2.687E+1 1.200E+1 8.907E+0 7.157E+0 0.000E+0 5.199E+0 7.808E-1
alpha_1 9.560E-1 9.784E-2 8.940E-1 1.165E-1 7.924E-1 2.401E-1 9.239E-1 8.803E-2 9.200E-1 1.110E-16 1.200E+0 0.000E+0
f_2 2.470E-1 8.860E-2 4.586E-1 2.445E-1 4.646E-2 8.002E-2 1.782E-1 6.859E-2 6.301E-1 3.761E-1 1.297E-1 1.835E-1
tauD_2 [ms] 3.769E+1 2.217E+1 6.512E+1 5.769E+1 6.824E+1 5.464E+1 2.987E+1 1.846E+1 8.895E+1 9.386E+1 1.059E+2 1.005E+2
D_2 [mum^2/s] 3.389E-1 1.244E-1 7.719E-1 1.070E+0 2.650E-1 1.383E-1 4.318E-1 1.642E-1 1.844E+0 2.075E+0 2.516E-1 1.520E-1
alpha_2 1.574E+0 3.177E-1 1.350E+0 3.675E-1 1.173E+0 2.218E-1 1.284E+0 2.347E-1 1.710E+0 3.721E-1 1.239E+0 2.424E-1
tauD_m [ms] 1.036E+1 5.561E+0 3.627E+1 3.706E+1 4.465E+0 8.115E+0 6.374E+0 5.130E+0 5.589E+1 6.804E+1 3.425E+1 4.485E+1
D_m [mum^2/s] 4.625E+0 1.505E+0 3.702E+0 1.556E+0 1.833E+1 2.678E+1 9.728E+0 5.591E+0 4.148E+0 2.324E+0 4.432E+0 7.858E-1
R [1/s] 1.198E+1 1.295E+1 9.581E+0 6.816E+0
CPM [kHz] 1.278E+0 3.662E-1 3.126E-1 2.539E-1 1.045E+0 4.198E-1 2.959E-1 2.168E-1
CPM_corr [kHz] 1.302E+0 3.675E-1 3.584E-1 2.681E-1 1.205E+0 4.645E-1 3.368E-1 2.205E-1
Int [kHz] 1.815E+2 1.289E+2 1.517E+2 3.183E+1 2.630E+1 4.146E+1 7.174E+1 5.086E+1
Int_corr [kHz] 2.355E+2 1.791E+2 2.363E+2 6.755E+1 3.478E+1 5.426E+1 1.054E+2 5.697E+1
# samples 105 105 25 25 16 16 150 150 5 5 3 3
# cells 54

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1098.142 - sd = 856.311Int_corr [kHz] - Ch1 - Nuc : ave = 235.536 - sd = 179.091r = 0.874 - r^2 = 0.764
f_1 - Ch1 - Nuc : ave = 0.753 - sd = 0.089D_1 [mum^2/s] - Ch1 - Nuc : ave = 6.109 - sd = 2.055r = -0.262 - r^2 = 0.068
f_2 - Ch1 - Nuc : ave = 0.247 - sd = 0.089D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.339 - sd = 0.124r = 0.403 - r^2 = 0.162
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.302 - sd = 0.368D_m [mum^2/s] - Ch1 - Nuc : ave = 4.625 - sd = 1.505r = 0.214 - r^2 = 0.046
c_corr [nM] - Ch1 - Nuc : ave = 1098.142 - sd = 856.311ratioG_corr - Cross - Nuc : ave = 0.023 - sd = 0.025r = 0.085 - r^2 = 0.007
c_corr [nM] - Ch1 - Cyto : ave = 204.758 - sd = 338.971Int_corr [kHz] - Ch1 - Cyto : ave = 34.782 - sd = 54.261r = 0.713 - r^2 = 0.509
f_1 - Ch1 - Cyto : ave = 0.822 - sd = 0.069D_1 [mum^2/s] - Ch1 - Cyto : ave = 11.999 - sd = 8.907r = -0.222 - r^2 = 0.049
f_2 - Ch1 - Cyto : ave = 0.178 - sd = 0.069D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.432 - sd = 0.164r = -0.104 - r^2 = 0.011
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.205 - sd = 0.464D_m [mum^2/s] - Ch1 - Cyto : ave = 9.728 - sd = 5.591r = 0.201 - r^2 = 0.040