Automated F(C)CS analysis of clone # 1246

Created on 12/08/2014
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Gene and protein information

Clone # 1246
Gene symbol: USP1
Description: ubiquitin specific peptidase 1
Molecular weight (with FP): 115.2 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1246_img_ch1.png1246_img_ch2.png


Point 1, intensity traces and correlation functions
1246_itr_1.png1246_cor_1.png

Point 2, intensity traces and correlation functions
1246_itr_2.png1246_cor_2.png

Point 3, intensity traces and correlation functions
1246_itr_3.png1246_cor_3.png

Point 4, intensity traces and correlation functions
1246_itr_4.png1246_cor_4.png

Point 5, intensity traces and correlation functions
1246_itr_5.png1246_cor_5.png

Point 6, intensity traces and correlation functions
1246_itr_6.png1246_cor_6.png

Sessions

1 20111104\U03--V00--1246
2 20111108\U02--V01--1246
3 20111109\U02--V00--1246
4 20111110\U02--V01--1246

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.494E+2 9.782E+1 1.318E+3 6.370E+2 1.990E+1 2.741E+1 2.130E+1 3.183E+1 3.095E+2 2.489E+2 3.536E+1 8.803E+1
N_corr 1.634E+2 1.083E+2 1.861E+3 1.045E+3 2.644E+1 6.079E+1 2.264E+1 4.470E+1 4.110E+2 5.088E+2 4.634E+1 1.122E+2
c [nM] 9.293E+2 6.084E+2 6.114E+3 2.955E+3 1.023E+2 1.409E+2 1.325E+2 1.980E+2 1.436E+3 1.154E+3 1.818E+2 4.526E+2
c_corr [nM] 1.016E+3 6.733E+2 8.633E+3 4.847E+3 1.359E+2 3.125E+2 1.408E+2 2.780E+2 1.906E+3 2.360E+3 2.382E+2 5.768E+2
ratioG 1.050E-1 8.719E-2 3.295E-1 3.437E-1
ratioG_corr 2.385E-2 1.147E-1 2.704E-1 4.504E-1
f_1 8.978E-1 6.560E-2 3.654E-1 2.412E-1 9.813E-1 5.849E-2 9.083E-1 5.965E-2 6.344E-1 3.739E-1 9.687E-1 7.837E-2
tauD_1 [ms] 1.903E+0 8.784E-1 1.860E+0 4.441E-16 1.139E+0 8.263E-1 5.495E-1 7.675E-1 1.860E+0 0.000E+0 1.953E+0 2.360E+0
D_1 [mum^2/s] 6.834E+0 3.796E+0 6.926E+0 5.329E-15 2.443E+1 2.851E+1 2.921E+1 1.498E+1 6.926E+0 0.000E+0 1.814E+1 2.616E+1
alpha_1 9.726E-1 8.931E-2 9.200E-1 1.110E-15 8.937E-1 2.404E-1 9.941E-1 1.157E-1 9.200E-1 1.110E-16 1.010E+0 2.748E-1
f_2 1.022E-1 6.560E-2 6.346E-1 2.412E-1 1.872E-2 5.849E-2 9.170E-2 5.965E-2 3.656E-1 3.739E-1 3.133E-2 7.837E-2
tauD_2 [ms] 2.631E+1 2.469E+1 6.345E+1 8.841E+1 8.036E+1 6.795E+1 1.458E+1 1.443E+1 7.665E+1 1.344E+2 5.346E+1 2.939E+1
D_2 [mum^2/s] 4.992E-1 1.976E-1 7.400E-1 8.971E-1 2.107E-1 1.653E-1 1.033E+0 5.084E-1 1.090E+0 1.385E+0 3.403E-1 3.620E-1
alpha_2 1.348E+0 3.438E-1 1.275E+0 3.145E-1 1.086E+0 1.286E-1 1.191E+0 2.856E-1 1.317E+0 4.348E-1 1.117E+0 1.516E-1
tauD_m [ms] 4.339E+0 2.926E+0 3.581E+1 4.905E+1 4.985E+0 2.075E+1 2.037E+0 3.619E+0 1.507E+1 2.400E+1 4.967E+0 1.085E+1
D_m [mum^2/s] 6.135E+0 3.288E+0 3.095E+0 1.386E+0 2.393E+1 2.782E+1 2.695E+1 1.480E+1 5.161E+0 1.693E+0 1.769E+1 2.611E+1
R [1/s] 2.018E+1 2.280E+1 4.315E+1 7.665E+1
CPM [kHz] 1.315E+0 3.573E-1 3.732E-1 3.040E-1 8.586E-1 3.884E-1 3.284E-1 2.393E-1
CPM_corr [kHz] 1.332E+0 3.577E-1 4.271E-1 3.465E-1 1.080E+0 4.424E-1 6.025E-1 9.858E-1
Int [kHz] 2.044E+2 1.200E+2 2.412E+2 7.148E+1 1.772E+1 1.911E+1 7.558E+1 5.287E+1
Int_corr [kHz] 2.226E+2 1.269E+2 3.805E+2 1.234E+2 2.109E+1 3.217E+1 1.103E+2 1.016E+2
# samples 507 507 423 423 356 356 400 400 40 40 28 28
# cells 187

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1016.167 - sd = 673.250Int_corr [kHz] - Ch1 - Nuc : ave = 222.580 - sd = 126.918r = 0.804 - r^2 = 0.647
f_1 - Ch1 - Nuc : ave = 0.898 - sd = 0.066D_1 [mum^2/s] - Ch1 - Nuc : ave = 6.834 - sd = 3.796r = -0.168 - r^2 = 0.028
f_2 - Ch1 - Nuc : ave = 0.102 - sd = 0.066D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.499 - sd = 0.198r = 0.101 - r^2 = 0.010
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.332 - sd = 0.358D_m [mum^2/s] - Ch1 - Nuc : ave = 6.135 - sd = 3.288r = 0.093 - r^2 = 0.009
c_corr [nM] - Ch1 - Nuc : ave = 1016.167 - sd = 673.250ratioG_corr - Cross - Nuc : ave = 0.024 - sd = 0.115r = 0.033 - r^2 = 0.001
c_corr [nM] - Ch1 - Cyto : ave = 140.821 - sd = 278.022Int_corr [kHz] - Ch1 - Cyto : ave = 21.090 - sd = 32.168r = 0.618 - r^2 = 0.382
f_1 - Ch1 - Cyto : ave = 0.908 - sd = 0.060D_1 [mum^2/s] - Ch1 - Cyto : ave = 29.206 - sd = 14.982r = 0.329 - r^2 = 0.108
f_2 - Ch1 - Cyto : ave = 0.092 - sd = 0.060D_2 [mum^2/s] - Ch1 - Cyto : ave = 1.033 - sd = 0.508r = -0.410 - r^2 = 0.168
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.080 - sd = 0.442D_m [mum^2/s] - Ch1 - Cyto : ave = 26.951 - sd = 14.804r = 0.389 - r^2 = 0.151