Automated F(C)CS analysis of clone # 1353

Created on 12/08/2014
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Gene and protein information

Clone # 1353
Gene symbol: SAP130
Description: Sin3A-associated protein, 130kDa
Molecular weight (with FP): 137.3 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1353_img_ch1.png1353_img_ch2.png


Point 1, intensity traces and correlation functions
1353_itr_1.png1353_cor_1.png

Point 2, intensity traces and correlation functions
1353_itr_2.png1353_cor_2.png

Point 3, intensity traces and correlation functions
1353_itr_3.png1353_cor_3.png

Point 4, intensity traces and correlation functions
1353_itr_4.png1353_cor_4.png

Point 5, intensity traces and correlation functions
1353_itr_5.png1353_cor_5.png

Point 6, intensity traces and correlation functions
1353_itr_6.png1353_cor_6.png

Sessions

1 20111108\U03--V01--1353
2 20111109\U01--V00--1353
3 20111110\U03--V01--1353
4 20111209\U02--V00--1353
5 20120117\U01--V00--1353

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.006E+2 5.815E+1 1.212E+3 5.979E+2 9.393E+0 1.021E+1 2.519E+1 4.202E+1 1.400E+2 1.564E+2 2.844E+1 5.811E+1
N_corr 1.058E+2 6.298E+1 1.859E+3 1.018E+3 1.064E+1 1.192E+1 2.416E+1 4.021E+1 1.668E+2 3.113E+2 3.032E+1 5.733E+1
c [nM] 6.258E+2 3.617E+2 5.622E+3 2.773E+3 4.829E+1 5.247E+1 1.567E+2 2.613E+2 6.492E+2 7.256E+2 1.462E+2 2.988E+2
c_corr [nM] 6.582E+2 3.917E+2 8.623E+3 4.724E+3 5.468E+1 6.126E+1 1.502E+2 2.501E+2 7.735E+2 1.444E+3 1.559E+2 2.948E+2
ratioG 7.389E-2 4.444E-2 4.935E-1 2.846E-1
ratioG_corr 4.673E-3 3.222E-2 1.695E-1 2.163E-1
f_1 8.528E-1 8.273E-2 3.341E-1 2.146E-1 9.725E-1 6.869E-2 9.175E-1 5.992E-2 7.526E-1 3.149E-1 9.586E-1 4.591E-2
tauD_1 [ms] 1.951E+0 1.154E+0 1.860E+0 2.220E-16 1.351E+0 1.221E+0 5.859E-1 5.477E-1 1.860E+0 4.441E-16 7.431E-1 8.922E-1
D_1 [mum^2/s] 8.242E+0 5.656E+0 6.926E+0 8.882E-16 2.300E+1 2.733E+1 2.663E+1 1.419E+1 6.926E+0 0.000E+0 4.935E+1 3.593E+1
alpha_1 9.710E-1 8.908E-2 9.200E-1 0.000E+0 8.512E-1 2.392E-1 1.007E+0 1.035E-1 9.200E-1 0.000E+0 9.211E-1 2.143E-1
f_2 1.472E-1 8.273E-2 6.659E-1 2.146E-1 2.748E-2 6.869E-2 8.250E-2 5.992E-2 2.474E-1 3.149E-1 4.145E-2 4.591E-2
tauD_2 [ms] 2.577E+1 1.255E+1 6.642E+1 6.963E+1 1.009E+2 7.703E+1 1.528E+1 1.814E+1 3.411E+1 4.047E+1 2.742E+1 2.152E+1
D_2 [mum^2/s] 4.957E-1 2.137E-1 4.926E-1 5.317E-1 1.679E-1 9.670E-2 1.078E+0 5.640E-1 1.010E+0 9.222E-1 7.966E-1 5.210E-1
alpha_2 1.394E+0 3.041E-1 1.276E+0 2.758E-1 1.117E+0 1.778E-1 1.080E+0 1.324E-1 1.240E+0 3.246E-1 1.051E+0 2.610E-1
tauD_m [ms] 5.518E+0 3.491E+0 4.345E+1 4.677E+1 6.356E+0 2.206E+1 2.085E+0 4.747E+0 9.725E+0 1.326E+1 1.664E+0 1.720E+0
D_m [mum^2/s] 7.006E+0 4.751E+0 2.669E+0 1.337E+0 2.263E+1 2.735E+1 2.451E+1 1.292E+1 5.598E+0 1.608E+0 4.682E+1 3.417E+1
R [1/s] 2.393E+1 2.309E+1 4.249E+1 4.163E+1
CPM [kHz] 1.414E+0 3.196E-1 3.375E-1 3.449E-1 1.068E+0 4.479E-1 1.801E-1 1.507E-1
CPM_corr [kHz] 1.438E+0 3.215E-1 3.798E-1 3.837E-1 1.211E+0 4.714E-1 6.344E-1 5.733E-1
Int [kHz] 1.540E+2 9.394E+1 2.247E+2 5.405E+1 2.359E+1 1.714E+1 2.991E+1 4.093E+1
Int_corr [kHz] 1.642E+2 1.020E+2 3.879E+2 1.155E+2 2.506E+1 1.827E+1 4.782E+1 9.113E+1
# samples 166 166 127 127 111 111 139 139 13 13 11 11
# cells 57

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 658.161 - sd = 391.657Int_corr [kHz] - Ch1 - Nuc : ave = 164.218 - sd = 102.025r = 0.914 - r^2 = 0.836
f_1 - Ch1 - Nuc : ave = 0.853 - sd = 0.083D_1 [mum^2/s] - Ch1 - Nuc : ave = 8.242 - sd = 5.656r = -0.377 - r^2 = 0.142
f_2 - Ch1 - Nuc : ave = 0.147 - sd = 0.083D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.496 - sd = 0.214r = 0.225 - r^2 = 0.051
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.438 - sd = 0.322D_m [mum^2/s] - Ch1 - Nuc : ave = 7.006 - sd = 4.751r = -0.049 - r^2 = 0.002
c_corr [nM] - Ch1 - Nuc : ave = 658.161 - sd = 391.657ratioG_corr - Cross - Nuc : ave = 0.005 - sd = 0.032r = 0.251 - r^2 = 0.063
c_corr [nM] - Ch1 - Cyto : ave = 150.243 - sd = 250.066Int_corr [kHz] - Ch1 - Cyto : ave = 25.062 - sd = 18.273r = 0.781 - r^2 = 0.610
f_1 - Ch1 - Cyto : ave = 0.917 - sd = 0.060D_1 [mum^2/s] - Ch1 - Cyto : ave = 26.626 - sd = 14.187r = 0.052 - r^2 = 0.003
f_2 - Ch1 - Cyto : ave = 0.083 - sd = 0.060D_2 [mum^2/s] - Ch1 - Cyto : ave = 1.078 - sd = 0.564r = 0.049 - r^2 = 0.002
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.211 - sd = 0.471D_m [mum^2/s] - Ch1 - Cyto : ave = 24.515 - sd = 12.922r = 0.404 - r^2 = 0.163