Automated F(C)CS analysis of clone # 1499

Created on 12/08/2014
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Gene and protein information

Clone # 1499
Gene symbol: PNMAL1
Description: paraneoplastic Ma antigen family-like 1
Molecular weight (with FP): 75.2 kDa
Localisation: homogeneous nucleus, bright nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1499_img_ch1.png1499_img_ch2.png


Point 1, intensity traces and correlation functions
1499_itr_1.png1499_cor_1.png

Point 2, intensity traces and correlation functions
1499_itr_2.png1499_cor_2.png

Point 3, intensity traces and correlation functions
1499_itr_3.png1499_cor_3.png

Point 4, intensity traces and correlation functions
1499_itr_4.png1499_cor_4.png

Point 5, intensity traces and correlation functions
1499_itr_5.png1499_cor_5.png

Point 6, intensity traces and correlation functions
1499_itr_6.png1499_cor_6.png

Sessions

1 20110914\U01--V00--1499
2 20111111\U03--V00--1499
3 20111115\U03--V00--1499
4 20111116\U03--V00--1499
5 20111130\U01--V00--1499
6 20111207\U00--V01--1499
7 20111213\U02--V00--1499
8 20120131\U02--V00--1499
9 20120222\U02--V00--1499
10 20120229\U01--V00--1499
11 20120309\U01--V00--1499
12 20120322\U01--V00--1499

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.429E+2 1.924E+2 1.099E+3 6.566E+2 2.535E+1 1.329E+2 2.760E+1 4.495E+1 2.843E+2 2.646E+2 5.959E+0 4.861E+0
N_corr 2.362E+2 4.045E+2 1.625E+3 1.078E+3 6.558E+1 3.154E+2 3.165E+1 6.051E+1 3.837E+2 4.124E+2 9.376E+0 9.486E+0
c [nM] 8.890E+2 1.196E+3 5.096E+3 3.045E+3 1.303E+2 6.831E+2 1.717E+2 2.796E+2 1.319E+3 1.227E+3 3.063E+1 2.499E+1
c_corr [nM] 1.469E+3 2.516E+3 7.539E+3 5.000E+3 3.371E+2 1.622E+3 1.968E+2 3.763E+2 1.780E+3 1.913E+3 4.820E+1 4.877E+1
ratioG 1.016E-1 1.353E-1 2.542E-1 2.008E-1
ratioG_corr 2.261E-2 1.670E-1 6.808E-2 5.696E-2
f_1 8.106E-1 1.630E-1 4.004E-1 2.137E-1 9.456E-1 9.527E-2 8.126E-1 1.058E-1 7.264E-1 2.744E-1 8.561E-1 9.379E-2
tauD_1 [ms] 3.952E+0 1.737E+0 1.838E+0 2.890E-2 3.365E+0 2.656E+0 2.708E+0 1.835E+0 6.412E+0 1.250E+1 2.416E+0 1.299E+0
D_1 [mum^2/s] 3.369E+0 2.245E+0 7.010E+0 1.112E-1 6.192E+0 7.705E+0 7.069E+0 1.170E+1 5.842E+0 2.261E+0 1.161E+1 2.057E+1
alpha_1 9.921E-1 1.027E-1 9.200E-1 2.596E-4 8.760E-1 1.920E-1 9.045E-1 1.128E-1 9.009E-1 1.453E-1 8.527E-1 2.298E-1
f_2 1.894E-1 1.630E-1 5.996E-1 2.137E-1 5.443E-2 9.527E-2 1.874E-1 1.058E-1 2.736E-1 2.744E-1 1.439E-1 9.379E-2
tauD_2 [ms] 4.337E+1 2.836E+1 9.173E+1 1.085E+2 9.528E+1 5.251E+1 4.547E+1 3.157E+1 7.377E+1 1.038E+2 7.049E+1 4.623E+1
D_2 [mum^2/s] 3.549E-1 2.984E-1 5.019E-1 7.375E-1 1.683E-1 1.310E-1 3.156E-1 1.731E-1 9.031E-1 1.017E+0 2.297E-1 1.198E-1
alpha_2 1.467E+0 3.249E-1 1.286E+0 3.206E-1 1.236E+0 2.962E-1 1.387E+0 2.996E-1 1.236E+0 3.488E-1 1.657E+0 3.911E-1
tauD_m [ms] 1.102E+1 9.402E+0 5.493E+1 7.297E+1 9.309E+0 1.600E+1 1.065E+1 8.896E+0 3.273E+1 3.876E+1 1.372E+1 1.574E+1
D_m [mum^2/s] 2.631E+0 1.174E+0 3.159E+0 1.322E+0 5.902E+0 7.595E+0 5.632E+0 8.457E+0 4.031E+0 2.088E+0 1.049E+1 2.004E+1
R [1/s] 1.834E+1 3.438E+1 4.588E+0 6.967E+0
CPM [kHz] 2.336E+0 9.262E-1 3.928E-1 3.540E-1 9.507E-1 7.153E-1 2.865E-1 2.102E-1
CPM_corr [kHz] 2.366E+0 9.311E-1 4.622E-1 4.135E-1 1.215E+0 7.584E-1 5.475E-1 5.096E-1
Int [kHz] 2.296E+2 1.727E+2 2.111E+2 6.790E+1 1.932E+1 2.526E+1 6.937E+1 6.455E+1
Int_corr [kHz] 3.481E+2 4.078E+2 3.628E+2 1.355E+2 2.423E+1 3.071E+1 1.117E+2 1.151E+2
# samples 209 209 175 175 167 167 187 187 31 31 22 22
# cells 127

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1469.032 - sd = 2515.980Int_corr [kHz] - Ch1 - Nuc : ave = 348.108 - sd = 407.762r = 0.757 - r^2 = 0.573
f_1 - Ch1 - Nuc : ave = 0.811 - sd = 0.163D_1 [mum^2/s] - Ch1 - Nuc : ave = 3.369 - sd = 2.245r = -0.478 - r^2 = 0.229
f_2 - Ch1 - Nuc : ave = 0.189 - sd = 0.163D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.355 - sd = 0.298r = -0.015 - r^2 = 0.000
CPM_corr [kHz] - Ch1 - Nuc : ave = 2.366 - sd = 0.931D_m [mum^2/s] - Ch1 - Nuc : ave = 2.631 - sd = 1.174r = -0.015 - r^2 = 0.000
c_corr [nM] - Ch1 - Nuc : ave = 1469.032 - sd = 2515.980ratioG_corr - Cross - Nuc : ave = 0.023 - sd = 0.167r = -0.213 - r^2 = 0.045
c_corr [nM] - Ch1 - Cyto : ave = 196.810 - sd = 376.346Int_corr [kHz] - Ch1 - Cyto : ave = 24.229 - sd = 30.711r = 0.541 - r^2 = 0.292
f_1 - Ch1 - Cyto : ave = 0.813 - sd = 0.106D_1 [mum^2/s] - Ch1 - Cyto : ave = 7.069 - sd = 11.697r = -0.006 - r^2 = 3.810E-5
f_2 - Ch1 - Cyto : ave = 0.187 - sd = 0.106D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.316 - sd = 0.173r = -0.050 - r^2 = 0.002
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.215 - sd = 0.758D_m [mum^2/s] - Ch1 - Cyto : ave = 5.632 - sd = 8.457r = -0.079 - r^2 = 0.006