Automated F(C)CS analysis of clone # 1838

Created on 12/08/2014
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Gene and protein information

Clone # 1838
Gene symbol: RPL10
Description: ribosomal protein L10
Molecular weight (with FP): 51.6 kDa
Localisation: homogeneous nucleus, bright nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1838_img_ch1.png1838_img_ch2.png


Point 1, intensity traces and correlation functions
1838_itr_1.png1838_cor_1.png

Point 2, intensity traces and correlation functions
1838_itr_2.png1838_cor_2.png

Point 3, intensity traces and correlation functions
1838_itr_3.png1838_cor_3.png

Point 4, intensity traces and correlation functions
1838_itr_4.png1838_cor_4.png

Point 5, intensity traces and correlation functions
1838_itr_5.png1838_cor_5.png

Point 6, intensity traces and correlation functions
1838_itr_6.png1838_cor_6.png

Sessions

1 20110901\U01--V00--1838
2 20110901\U02--V00--1838
3 20110901\U03--V00--1838
4 20110902\U02--V00--1838
5 20110906\U01--V00--1838
6 20110907\U03--V00--1838
7 20110908\U03--V00--1838

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 3.012E+1 1.976E+1 8.680E+2 3.717E+2 1.577E+0 1.582E+0 6.758E+1 4.721E+1 2.394E+2 2.094E+2 4.512E+1 3.868E+1
N_corr 3.193E+1 2.234E+1 1.406E+3 7.045E+2 1.967E+0 2.072E+0 9.487E+1 7.011E+1 2.554E+2 4.435E+2 8.700E+1 7.488E+1
c [nM] 1.873E+2 1.229E+2 4.026E+3 1.724E+3 8.108E+0 8.133E+0 4.203E+2 2.936E+2 1.110E+3 9.713E+2 2.320E+2 1.989E+2
c_corr [nM] 1.986E+2 1.390E+2 6.524E+3 3.268E+3 1.011E+1 1.065E+1 5.900E+2 4.360E+2 1.185E+3 2.057E+3 4.473E+2 3.849E+2
ratioG 3.999E-2 2.213E-2 5.468E-1 2.481E-1
ratioG_corr 5.877E-3 1.459E-2 1.064E-1 9.462E-2
f_1 8.481E-1 7.478E-2 4.630E-1 2.216E-1 9.813E-1 4.995E-2 7.909E-1 1.093E-1 1.000E+0 0.000E+0 8.521E-1 1.118E-1
tauD_1 [ms] 1.172E+0 8.345E-1 1.835E+0 8.996E-2 8.946E-1 1.098E+0 2.624E+0 2.028E+0 7.918E+0 1.037E+1 1.395E+0 1.429E+0
D_1 [mum^2/s] 1.249E+1 6.365E+0 7.042E+0 4.566E-1 3.111E+1 2.944E+1 5.762E+0 3.602E+0 3.693E+0 3.016E+0 1.826E+1 2.034E+1
alpha_1 9.856E-1 1.167E-1 9.337E-1 3.327E-2 8.845E-1 2.362E-1 8.826E-1 2.200E-1 8.478E-1 2.308E-1 6.832E-1 1.472E-1
f_2 1.519E-1 7.478E-2 5.370E-1 2.216E-1 1.867E-2 4.995E-2 2.091E-1 1.093E-1 0.000E+0 0.000E+0 1.479E-1 1.118E-1
tauD_2 [ms] 2.030E+1 1.406E+1 1.010E+2 1.341E+2 8.108E+1 5.041E+1 6.316E+1 6.030E+1 1.358E+1 2.151E+1 8.199E+1 1.685E+2
D_2 [mum^2/s] 7.031E-1 3.424E-1 5.718E-1 8.937E-1 1.929E-1 9.337E-2 2.570E-1 1.415E-1 2.249E+0 8.211E-1 2.465E-1 1.252E-1
alpha_2 1.405E+0 3.290E-1 1.406E+0 3.573E-1 1.142E+0 1.302E-1 1.486E+0 3.569E-1 1.041E+0 6.184E-2 1.439E+0 3.131E-1
tauD_m [ms] 4.364E+0 3.880E+0 5.286E+1 7.115E+1 3.112E+0 9.289E+0 1.360E+1 1.529E+1 7.918E+0 1.037E+1 1.260E+1 2.399E+1
D_m [mum^2/s] 1.087E+1 5.896E+0 3.637E+0 1.322E+0 3.066E+1 2.920E+1 4.594E+0 2.954E+0 3.693E+0 3.016E+0 1.513E+1 1.699E+1
R [1/s] 6.065E+1 4.585E+1 1.934E+0 6.688E+0
CPM [kHz] 1.194E+0 2.942E-1 3.314E-1 2.878E-1 1.200E+0 5.732E-1 1.998E-1 1.496E-1
CPM_corr [kHz] 1.282E+0 2.883E-1 3.497E-1 3.034E-1 1.245E+0 5.931E-1 8.444E-1 1.229E+0
Int [kHz] 4.140E+1 2.928E+1 1.369E+2 3.903E+1 7.778E+1 6.129E+1 3.986E+1 3.726E+1
Int_corr [kHz] 4.596E+1 3.241E+1 2.289E+2 7.397E+1 1.102E+2 8.333E+1 6.497E+1 7.603E+1
# samples 576 576 358 358 179 179 953 953 284 284 243 243
# cells 325

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 198.552 - sd = 138.963Int_corr [kHz] - Ch1 - Nuc : ave = 45.956 - sd = 32.413r = 0.864 - r^2 = 0.747
f_1 - Ch1 - Nuc : ave = 0.848 - sd = 0.075D_1 [mum^2/s] - Ch1 - Nuc : ave = 12.490 - sd = 6.365r = 0.402 - r^2 = 0.161
f_2 - Ch1 - Nuc : ave = 0.152 - sd = 0.075D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.703 - sd = 0.342r = -0.437 - r^2 = 0.191
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.282 - sd = 0.288D_m [mum^2/s] - Ch1 - Nuc : ave = 10.869 - sd = 5.896r = 0.030 - r^2 = 0.001
c_corr [nM] - Ch1 - Nuc : ave = 198.552 - sd = 138.963ratioG_corr - Cross - Nuc : ave = 0.006 - sd = 0.015r = -0.158 - r^2 = 0.025
c_corr [nM] - Ch1 - Cyto : ave = 590.026 - sd = 436.002Int_corr [kHz] - Ch1 - Cyto : ave = 110.193 - sd = 83.327r = 0.659 - r^2 = 0.434
f_1 - Ch1 - Cyto : ave = 0.791 - sd = 0.109D_1 [mum^2/s] - Ch1 - Cyto : ave = 5.762 - sd = 3.602r = 0.008 - r^2 = 5.987E-5
f_2 - Ch1 - Cyto : ave = 0.209 - sd = 0.109D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.257 - sd = 0.142r = 0.084 - r^2 = 0.007
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.245 - sd = 0.593D_m [mum^2/s] - Ch1 - Cyto : ave = 4.594 - sd = 2.954r = -0.234 - r^2 = 0.055