Automated F(C)CS analysis of clone # 1909

Created on 12/08/2014
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Gene and protein information

Clone # 1909
Gene symbol: DMAP1
Description: DNA methyltransferase 1 associated protein 1
Molecular weight (with FP): 80.0 kDa
Localisation: granular nucleus, ave. nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1909_img_ch1.png1909_img_ch2.png


Point 1, intensity traces and correlation functions
1909_itr_1.png1909_cor_1.png

Point 2, intensity traces and correlation functions
1909_itr_2.png1909_cor_2.png

Point 3, intensity traces and correlation functions
1909_itr_3.png1909_cor_3.png

Point 4, intensity traces and correlation functions
1909_itr_4.png1909_cor_4.png

Point 5, intensity traces and correlation functions
1909_itr_5.png1909_cor_5.png

Point 6, intensity traces and correlation functions
1909_itr_6.png1909_cor_6.png

Sessions

1 20111111\U01--V01--1909
2 20111115\U01--V01--1909
3 20111116\U01--V01--1909
4 20111215\U01--V00--1909

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.112E+2 4.974E+1 1.237E+3 5.433E+2 1.034E+1 8.259E+0 2.354E+1 2.177E+1 2.107E+2 1.972E+2 1.407E+1 8.876E+0
N_corr 1.197E+2 5.491E+1 1.792E+3 9.087E+2 1.221E+1 1.009E+1 2.471E+1 2.733E+1 2.814E+2 3.912E+2 2.030E+1 1.658E+1
c [nM] 6.916E+2 3.094E+2 5.738E+3 2.520E+3 5.317E+1 4.246E+1 1.464E+2 1.354E+2 9.771E+2 9.147E+2 7.235E+1 4.563E+1
c_corr [nM] 7.446E+2 3.415E+2 8.313E+3 4.215E+3 6.276E+1 5.185E+1 1.537E+2 1.700E+2 1.305E+3 1.814E+3 1.044E+2 8.525E+1
ratioG 8.252E-2 4.500E-2 4.157E-1 2.426E-1
ratioG_corr 3.179E-3 3.200E-2 1.014E-1 7.935E-2
f_1 8.691E-1 1.120E-1 3.644E-1 1.952E-1 9.668E-1 7.374E-2 8.760E-1 7.643E-2 6.748E-1 2.649E-1 9.173E-1 9.052E-2
tauD_1 [ms] 2.608E+0 1.638E+0 1.860E+0 4.441E-16 2.224E+0 3.885E+0 1.349E+0 1.035E+0 1.860E+0 2.220E-16 1.479E+0 7.225E-1
D_1 [mum^2/s] 5.965E+0 4.105E+0 6.926E+0 8.882E-16 1.203E+1 1.609E+1 1.286E+1 1.054E+1 6.926E+0 0.000E+0 1.038E+1 5.854E+0
alpha_1 9.458E-1 8.969E-2 9.203E-1 7.030E-4 8.605E-1 2.088E-1 9.170E-1 1.264E-1 9.202E-1 5.528E-4 7.789E-1 2.000E-1
f_2 1.309E-1 1.120E-1 6.356E-1 1.952E-1 3.319E-2 7.374E-2 1.240E-1 7.643E-2 3.252E-1 2.649E-1 8.265E-2 9.052E-2
tauD_2 [ms] 3.134E+1 1.860E+1 7.770E+1 8.423E+1 1.158E+2 2.582E+2 3.037E+1 3.414E+1 7.332E+1 8.032E+1 5.387E+1 3.872E+1
D_2 [mum^2/s] 4.122E-1 1.620E-1 5.126E-1 6.872E-1 1.842E-1 2.692E-1 5.277E-1 3.081E-1 5.488E-1 7.492E-1 2.967E-1 1.295E-1
alpha_2 1.357E+0 2.857E-1 1.289E+0 2.873E-1 1.152E+0 2.118E-1 1.211E+0 2.097E-1 1.194E+0 3.138E-1 1.293E+0 3.084E-1
tauD_m [ms] 6.381E+0 5.311E+0 4.951E+1 6.133E+1 8.607E+0 2.645E+1 5.427E+0 1.182E+1 2.460E+1 4.122E+1 6.379E+0 9.625E+0
D_m [mum^2/s] 5.061E+0 3.260E+0 2.904E+0 1.165E+0 1.150E+1 1.484E+1 1.132E+1 9.581E+0 4.964E+0 1.474E+0 9.499E+0 5.265E+0
R [1/s] 1.865E+1 1.999E+1 1.782E+1 2.813E+1
CPM [kHz] 1.547E+0 5.862E-1 4.128E-1 3.524E-1 1.143E+0 4.490E-1 2.621E-1 3.588E-1
CPM_corr [kHz] 1.567E+0 5.859E-1 4.728E-1 3.977E-1 1.272E+0 4.515E-1 6.231E-1 4.502E-1
Int [kHz] 1.863E+2 1.018E+2 2.214E+2 6.112E+1 2.877E+1 2.532E+1 4.453E+1 5.579E+1
Int_corr [kHz] 2.023E+2 1.111E+2 3.623E+2 1.088E+2 3.243E+1 3.096E+1 7.861E+1 1.136E+2
# samples 225 225 187 187 176 176 200 200 36 36 29 29
# cells 80

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 744.632 - sd = 341.518Int_corr [kHz] - Ch1 - Nuc : ave = 202.267 - sd = 111.118r = 0.893 - r^2 = 0.797
f_1 - Ch1 - Nuc : ave = 0.869 - sd = 0.112D_1 [mum^2/s] - Ch1 - Nuc : ave = 5.965 - sd = 4.105r = -0.459 - r^2 = 0.210
f_2 - Ch1 - Nuc : ave = 0.131 - sd = 0.112D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.412 - sd = 0.162r = 0.179 - r^2 = 0.032
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.567 - sd = 0.586D_m [mum^2/s] - Ch1 - Nuc : ave = 5.061 - sd = 3.260r = -0.460 - r^2 = 0.212
c_corr [nM] - Ch1 - Nuc : ave = 744.632 - sd = 341.518ratioG_corr - Cross - Nuc : ave = 0.003 - sd = 0.032r = 0.109 - r^2 = 0.012
c_corr [nM] - Ch1 - Cyto : ave = 153.707 - sd = 169.988Int_corr [kHz] - Ch1 - Cyto : ave = 32.425 - sd = 30.959r = 0.791 - r^2 = 0.626
f_1 - Ch1 - Cyto : ave = 0.876 - sd = 0.076D_1 [mum^2/s] - Ch1 - Cyto : ave = 12.857 - sd = 10.539r = -0.343 - r^2 = 0.118
f_2 - Ch1 - Cyto : ave = 0.124 - sd = 0.076D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.528 - sd = 0.308r = 0.181 - r^2 = 0.033
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.272 - sd = 0.451D_m [mum^2/s] - Ch1 - Cyto : ave = 11.324 - sd = 9.581r = -0.249 - r^2 = 0.062