Automated F(C)CS analysis of clone # 1947

Created on 12/08/2014
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Gene and protein information

Clone # 1947
Gene symbol: SMARCE1
Description: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Molecular weight (with FP): 73.6 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
1947_img_ch1.png1947_img_ch2.png


Point 1, intensity traces and correlation functions
1947_itr_1.png1947_cor_1.png

Point 2, intensity traces and correlation functions
1947_itr_2.png1947_cor_2.png

Point 3, intensity traces and correlation functions
1947_itr_3.png1947_cor_3.png

Point 4, intensity traces and correlation functions
1947_itr_4.png1947_cor_4.png

Point 5, intensity traces and correlation functions
1947_itr_5.png1947_cor_5.png

Point 6, intensity traces and correlation functions
1947_itr_6.png1947_cor_6.png

Sessions

1 20110915\U01--V01--1947
2 20110916\U01--V01--1947
3 20110920\U01--V00--1947
4 20111208\U01--V00--1947
5 20120116\U01--V00--1947
6 20120127\U00--V00--1947

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 8.713E+1 6.664E+1 1.101E+3 6.770E+2 9.834E+0 1.860E+1 2.735E+1 3.284E+1 5.509E+2 3.056E+2 2.220E+1 3.380E+1
N_corr 9.220E+1 6.845E+1 1.591E+3 1.138E+3 1.217E+1 2.695E+1 2.795E+1 3.681E+1 7.124E+2 5.332E+2 2.184E+1 3.254E+1
c [nM] 5.419E+2 4.144E+2 5.108E+3 3.140E+3 5.055E+1 9.563E+1 1.701E+2 2.043E+2 2.555E+3 1.417E+3 1.141E+2 1.738E+2
c_corr [nM] 5.734E+2 4.257E+2 7.379E+3 5.278E+3 6.256E+1 1.385E+2 1.738E+2 2.289E+2 3.304E+3 2.473E+3 1.123E+2 1.673E+2
ratioG 8.475E-2 7.744E-2 2.623E-1 2.200E-1
ratioG_corr 1.881E-2 7.494E-2 9.416E-2 1.300E-1
f_1 8.820E-1 6.976E-2 3.614E-1 2.250E-1 9.737E-1 6.477E-2 9.172E-1 6.020E-2 7.036E-1 2.804E-1 9.447E-1 4.040E-2
tauD_1 [ms] 1.369E+0 5.710E-1 1.839E+0 2.867E-2 1.060E+0 7.620E-1 6.295E-1 4.604E-1 2.453E+0 1.551E+0 1.360E+0 9.286E-1
D_1 [mum^2/s] 9.102E+0 3.778E+0 7.007E+0 1.103E-1 1.914E+1 1.768E+1 2.379E+1 1.158E+1 6.268E+0 1.664E+0 1.890E+1 1.735E+1
alpha_1 9.489E-1 7.488E-2 9.200E-1 0.000E+0 8.933E-1 2.764E-1 1.029E+0 1.226E-1 9.031E-1 4.232E-2 1.105E+0 1.824E-1
f_2 1.180E-1 6.976E-2 6.386E-1 2.250E-1 2.628E-2 6.477E-2 8.282E-2 6.020E-2 2.964E-1 2.804E-1 5.531E-2 4.040E-2
tauD_2 [ms] 2.144E+1 7.243E+0 8.471E+1 8.203E+1 7.812E+1 4.649E+1 1.871E+1 2.752E+1 3.279E+1 3.364E+1 4.751E+1 2.556E+1
D_2 [mum^2/s] 5.443E-1 1.638E-1 4.869E-1 7.750E-1 2.017E-1 9.813E-2 9.643E-1 5.330E-1 1.794E+0 2.212E+0 4.217E-1 3.854E-1
alpha_2 1.299E+0 2.214E-1 1.302E+0 3.187E-1 1.093E+0 9.648E-2 1.103E+0 1.600E-1 1.136E+0 2.734E-1 1.049E+0 2.568E-1
tauD_m [ms] 3.766E+0 2.368E+0 5.281E+1 5.406E+1 3.682E+0 7.609E+0 2.277E+0 3.721E+0 1.112E+1 1.125E+1 3.654E+0 2.877E+0
D_m [mum^2/s] 8.076E+0 3.249E+0 2.875E+0 1.479E+0 1.878E+1 1.763E+1 2.195E+1 1.092E+1 4.855E+0 1.656E+0 1.755E+1 1.567E+1
R [1/s] 3.647E+1 2.580E+1 3.412E+1 4.070E+1
CPM [kHz] 1.338E+0 3.618E-1 3.418E-1 2.777E-1 9.178E-1 4.705E-1 1.994E-1 7.477E-2
CPM_corr [kHz] 1.370E+0 3.629E-1 3.862E-1 3.102E-1 1.088E+0 5.010E-1 3.249E-1 2.479E-1
Int [kHz] 1.213E+2 7.936E+1 1.780E+2 7.617E+1 2.339E+1 3.154E+1 8.807E+1 4.478E+1
Int_corr [kHz] 1.308E+2 8.517E+1 2.832E+2 1.494E+2 2.526E+1 3.221E+1 1.298E+2 8.830E+1
# samples 103 103 85 85 72 72 89 89 13 13 9 9
# cells 41

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 573.421 - sd = 425.734Int_corr [kHz] - Ch1 - Nuc : ave = 130.816 - sd = 85.168r = 0.940 - r^2 = 0.883
f_1 - Ch1 - Nuc : ave = 0.882 - sd = 0.070D_1 [mum^2/s] - Ch1 - Nuc : ave = 9.102 - sd = 3.778r = -0.037 - r^2 = 0.001
f_2 - Ch1 - Nuc : ave = 0.118 - sd = 0.070D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.544 - sd = 0.164r = -0.295 - r^2 = 0.087
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.370 - sd = 0.363D_m [mum^2/s] - Ch1 - Nuc : ave = 8.076 - sd = 3.249r = 0.009 - r^2 = 8.193E-5
c_corr [nM] - Ch1 - Nuc : ave = 573.421 - sd = 425.734ratioG_corr - Cross - Nuc : ave = 0.019 - sd = 0.075r = 0.139 - r^2 = 0.019
c_corr [nM] - Ch1 - Cyto : ave = 173.818 - sd = 228.907Int_corr [kHz] - Ch1 - Cyto : ave = 25.260 - sd = 32.209r = 0.785 - r^2 = 0.616
f_1 - Ch1 - Cyto : ave = 0.917 - sd = 0.060D_1 [mum^2/s] - Ch1 - Cyto : ave = 23.792 - sd = 11.580r = -0.116 - r^2 = 0.013
f_2 - Ch1 - Cyto : ave = 0.083 - sd = 0.060D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.964 - sd = 0.533r = 0.138 - r^2 = 0.019
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.088 - sd = 0.501D_m [mum^2/s] - Ch1 - Cyto : ave = 21.945 - sd = 10.915r = 0.447 - r^2 = 0.200