Automated F(C)CS analysis of clone # 2002

Created on 12/08/2014
Back to overview

Gene and protein information

Clone # 2002
Gene symbol: DKC1
Description: dyskeratosis congenita 1, dyskerin
Molecular weight (with FP): 84.7 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2002_img_ch1.png2002_img_ch2.png


Point 1, intensity traces and correlation functions
2002_itr_1.png2002_cor_1.png

Point 2, intensity traces and correlation functions
2002_itr_2.png2002_cor_2.png

Point 3, intensity traces and correlation functions
2002_itr_3.png2002_cor_3.png

Point 4, intensity traces and correlation functions
2002_itr_4.png2002_cor_4.png

Point 5, intensity traces and correlation functions
2002_itr_5.png2002_cor_5.png

Point 6, intensity traces and correlation functions
2002_itr_6.png2002_cor_6.png

Sessions

1 20111117\U01--V00--2002
2 20111129\U00--V01--2002
3 20111206\U00--V01--2002
4 20111207\U01--V01--2002
5 20111216\U01--V00--2002

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.458E+2 7.633E+1 1.561E+3 7.121E+2 1.609E+1 1.527E+1 3.426E+1 3.494E+1 1.307E+2 1.584E+2 2.612E+1 5.206E+1
N_corr 1.692E+2 9.231E+1 2.281E+3 1.212E+3 1.893E+1 1.754E+1 3.568E+1 3.790E+1 1.087E+2 2.271E+2 3.400E+1 6.975E+1
c [nM] 9.069E+2 4.747E+2 7.238E+3 3.303E+3 8.271E+1 7.850E+1 2.131E+2 2.173E+2 6.062E+2 7.350E+2 1.343E+2 2.676E+2
c_corr [nM] 1.052E+3 5.741E+2 1.058E+4 5.623E+3 9.732E+1 9.016E+1 2.219E+2 2.357E+2 5.043E+2 1.053E+3 1.748E+2 3.586E+2
ratioG 9.284E-2 5.376E-2 5.242E-1 2.579E-1
ratioG_corr 6.047E-3 3.789E-2 1.342E-1 1.469E-1
f_1 8.323E-1 1.610E-1 3.598E-1 2.070E-1 9.732E-1 7.002E-2 8.667E-1 1.232E-1 7.012E-1 3.199E-1 9.424E-1 6.783E-2
tauD_1 [ms] 2.750E+0 1.700E+0 1.860E+0 2.220E-16 1.459E+0 3.106E+0 1.934E+0 1.419E+0 1.860E+0 2.220E-16 1.507E+0 1.438E+0
D_1 [mum^2/s] 5.020E+0 4.098E+0 6.926E+0 3.553E-15 1.950E+1 2.370E+1 7.814E+0 9.340E+0 6.926E+0 8.882E-16 1.179E+1 7.578E+0
alpha_1 9.468E-1 1.010E-1 9.200E-1 8.882E-16 8.774E-1 2.367E-1 8.992E-1 1.131E-1 9.200E-1 1.110E-16 7.693E-1 1.836E-1
f_2 1.677E-1 1.610E-1 6.402E-1 2.070E-1 2.676E-2 7.002E-2 1.333E-1 1.232E-1 2.988E-1 3.199E-1 5.758E-2 6.783E-2
tauD_2 [ms] 3.444E+1 2.598E+1 7.778E+1 9.412E+1 9.745E+1 9.807E+1 3.616E+1 3.076E+1 5.224E+1 7.651E+1 4.676E+1 3.176E+1
D_2 [mum^2/s] 4.960E-1 7.482E-1 5.324E-1 6.548E-1 1.992E-1 1.731E-1 6.825E-1 2.031E+0 8.021E-1 1.048E+0 3.554E-1 1.748E-1
alpha_2 1.354E+0 3.082E-1 1.242E+0 2.813E-1 1.114E+0 1.703E-1 1.197E+0 2.148E-1 1.244E+0 3.275E-1 1.280E+0 2.879E-1
tauD_m [ms] 8.036E+0 7.781E+0 4.925E+1 6.492E+1 7.242E+0 2.866E+1 6.054E+0 7.736E+0 1.211E+1 1.936E+1 4.413E+0 6.912E+0
D_m [mum^2/s] 4.189E+0 3.001E+0 2.877E+0 1.249E+0 1.908E+1 2.318E+1 6.884E+0 6.967E+0 5.283E+0 1.679E+0 1.114E+1 7.259E+0
R [1/s] 2.260E+1 4.054E+1 7.200E+0 1.207E+1
CPM [kHz] 1.276E+0 3.289E-1 3.983E-1 4.216E-1 1.086E+0 5.007E-1 1.641E-1 1.007E-1
CPM_corr [kHz] 1.292E+0 3.292E-1 4.540E-1 4.689E-1 1.194E+0 5.292E-1 2.308E-3 5.081E+0
Int [kHz] 1.932E+2 1.092E+2 2.412E+2 8.126E+1 3.339E+1 2.802E+1 2.474E+1 3.594E+1
Int_corr [kHz] 2.209E+2 1.167E+2 3.971E+2 1.482E+2 3.731E+1 3.047E+1 3.201E+1 5.163E+1
# samples 499 499 338 338 269 269 394 394 91 91 85 85
# cells 172

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1051.990 - sd = 574.124Int_corr [kHz] - Ch1 - Nuc : ave = 220.888 - sd = 116.656r = 0.815 - r^2 = 0.665
f_1 - Ch1 - Nuc : ave = 0.832 - sd = 0.161D_1 [mum^2/s] - Ch1 - Nuc : ave = 5.020 - sd = 4.098r = -0.017 - r^2 = 0.000
f_2 - Ch1 - Nuc : ave = 0.168 - sd = 0.161D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.496 - sd = 0.748r = -0.213 - r^2 = 0.045
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.292 - sd = 0.329D_m [mum^2/s] - Ch1 - Nuc : ave = 4.189 - sd = 3.001r = 0.258 - r^2 = 0.067
c_corr [nM] - Ch1 - Nuc : ave = 1051.990 - sd = 574.124ratioG_corr - Cross - Nuc : ave = 0.006 - sd = 0.038r = 0.228 - r^2 = 0.052
c_corr [nM] - Ch1 - Cyto : ave = 221.892 - sd = 235.728Int_corr [kHz] - Ch1 - Cyto : ave = 37.313 - sd = 30.475r = 0.656 - r^2 = 0.431
f_1 - Ch1 - Cyto : ave = 0.867 - sd = 0.123D_1 [mum^2/s] - Ch1 - Cyto : ave = 7.814 - sd = 9.340r = -0.329 - r^2 = 0.108
f_2 - Ch1 - Cyto : ave = 0.133 - sd = 0.123D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.683 - sd = 2.031r = 0.237 - r^2 = 0.056
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.194 - sd = 0.529D_m [mum^2/s] - Ch1 - Cyto : ave = 6.884 - sd = 6.967r = 0.269 - r^2 = 0.073