Automated F(C)CS analysis of clone # 2093hi

Created on 12/08/2014
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Gene and protein information

Clone # 2093hi
Gene symbol: MECP2
Description: methyl CpG binding protein 2 (Rett syndrome)
Molecular weight (with FP): 79.4 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2093hi_img_ch1.png2093hi_img_ch2.png


Point 1, intensity traces and correlation functions
2093hi_itr_1.png2093hi_cor_1.png

Point 2, intensity traces and correlation functions
2093hi_itr_2.png2093hi_cor_2.png

Point 3, intensity traces and correlation functions
2093hi_itr_3.png2093hi_cor_3.png

Point 4, intensity traces and correlation functions
2093hi_itr_4.png2093hi_cor_4.png

Point 5, intensity traces and correlation functions
2093hi_itr_5.png2093hi_cor_5.png

Point 6, intensity traces and correlation functions
2093hi_itr_6.png2093hi_cor_6.png

Sessions

1 20130125\U02--V01--2093hi
2 20130131\U01--V00--2093hi
3 20130131\U01--V01--2093hi
4 20130201\U01--V00--2093hi

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 3.822E+2 2.117E+2 9.389E+2 4.685E+2 1.120E+2 8.827E+1 3.129E+1 3.535E+1 3.099E+2 0.000E+0 7.727E+1 0.000E+0
N_corr 4.873E+2 3.042E+2 1.386E+3 8.373E+2 1.841E+2 1.613E+2 2.677E+1 3.729E+1 4.052E+2 0.000E+0 9.001E+1 0.000E+0
c [nM] 2.377E+3 1.317E+3 4.355E+3 2.173E+3 5.758E+2 4.538E+2 1.946E+2 2.198E+2 1.437E+3 0.000E+0 3.973E+2 0.000E+0
c_corr [nM] 3.031E+3 1.892E+3 6.429E+3 3.884E+3 9.466E+2 8.293E+2 1.665E+2 2.319E+2 1.880E+3 0.000E+0 4.627E+2 0.000E+0
ratioG 2.828E-1 1.330E-1 3.645E-1 0.000E+0
ratioG_corr 2.167E-1 1.091E-1 2.935E-1 0.000E+0
f_1 7.252E-1 2.235E-1 2.388E-1 1.470E-1 7.661E-1 2.056E-1 9.322E-1 7.026E-2 3.193E-1 0.000E+0 5.823E-1 0.000E+0
tauD_1 [ms] 8.795E+0 8.162E+0 1.860E+0 2.220E-16 1.240E+1 1.231E+1 5.840E-1 1.566E+0 1.860E+0 0.000E+0 1.213E+0 0.000E+0
D_1 [mum^2/s] 3.523E+0 3.904E+0 6.926E+0 8.882E-16 5.033E+0 1.155E+1 3.923E+1 2.194E+1 6.926E+0 0.000E+0 9.887E+0 0.000E+0
alpha_1 9.535E-1 1.400E-1 9.200E-1 0.000E+0 9.140E-1 2.091E-1 1.051E+0 1.751E-1 9.200E-1 0.000E+0 1.200E+0 0.000E+0
f_2 2.748E-1 2.235E-1 7.612E-1 1.470E-1 2.339E-1 2.056E-1 6.779E-2 7.026E-2 6.807E-1 0.000E+0 4.177E-1 0.000E+0
tauD_2 [ms] 8.613E+1 5.501E+1 6.427E+1 4.924E+1 1.401E+2 7.201E+1 2.177E+1 3.475E+1 1.235E+2 0.000E+0 6.905E+1 0.000E+0
D_2 [mum^2/s] 1.604E-1 7.429E-2 3.069E-1 1.914E-1 1.137E-1 7.363E-2 9.118E-1 5.151E-1 1.043E-1 0.000E+0 1.736E-1 0.000E+0
alpha_2 1.507E+0 3.672E-1 1.410E+0 2.896E-1 1.542E+0 4.111E-1 1.088E+0 1.183E-1 8.971E-1 0.000E+0 2.000E+0 0.000E+0
tauD_m [ms] 2.623E+1 1.284E+1 4.927E+1 4.225E+1 3.788E+1 2.327E+1 2.385E+0 4.257E+0 8.467E+1 0.000E+0 2.955E+1 0.000E+0
D_m [mum^2/s] 2.197E+0 2.494E+0 1.894E+0 9.501E-1 3.305E+0 7.921E+0 3.713E+1 2.190E+1 2.283E+0 0.000E+0 5.830E+0 0.000E+0
R [1/s] 4.929E-1 1.548E+0 5.627E-1 0.000E+0
CPM [kHz] 4.910E-1 2.543E-1 3.332E-1 2.833E-1 2.910E-1 1.237E-1 9.613E-1 0.000E+0
CPM_corr [kHz] 4.985E-1 2.534E-1 4.120E-1 3.609E-1 4.640E-1 2.552E-1 1.140E+0 0.000E+0
Int [kHz] 1.677E+2 1.067E+2 1.435E+2 4.385E+1 1.033E+1 1.855E+1 1.333E+2 0.000E+0
Int_corr [kHz] 2.155E+2 1.426E+2 2.513E+2 8.525E+1 1.099E+1 2.024E+1 2.068E+2 0.000E+0
# samples 106 106 68 68 62 62 59 59 1 1 1 1
# cells 39

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 3030.573 - sd = 1891.599Int_corr [kHz] - Ch1 - Nuc : ave = 215.549 - sd = 142.573r = 0.895 - r^2 = 0.801
f_1 - Ch1 - Nuc : ave = 0.725 - sd = 0.223D_1 [mum^2/s] - Ch1 - Nuc : ave = 3.523 - sd = 3.904r = -0.685 - r^2 = 0.469
f_2 - Ch1 - Nuc : ave = 0.275 - sd = 0.223D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.160 - sd = 0.074r = 0.597 - r^2 = 0.357
CPM_corr [kHz] - Ch1 - Nuc : ave = 0.499 - sd = 0.253D_m [mum^2/s] - Ch1 - Nuc : ave = 2.197 - sd = 2.494r = -0.332 - r^2 = 0.110
c_corr [nM] - Ch1 - Nuc : ave = 3030.573 - sd = 1891.599ratioG_corr - Cross - Nuc : ave = 0.217 - sd = 0.109r = 0.352 - r^2 = 0.124
c_corr [nM] - Ch1 - Cyto : ave = 166.500 - sd = 231.903Int_corr [kHz] - Ch1 - Cyto : ave = 10.992 - sd = 20.244r = 0.771 - r^2 = 0.595
f_1 - Ch1 - Cyto : ave = 0.932 - sd = 0.070D_1 [mum^2/s] - Ch1 - Cyto : ave = 39.228 - sd = 21.939r = 0.368 - r^2 = 0.135
f_2 - Ch1 - Cyto : ave = 0.068 - sd = 0.070D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.912 - sd = 0.515r = 0.094 - r^2 = 0.009
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.464 - sd = 0.255D_m [mum^2/s] - Ch1 - Cyto : ave = 37.134 - sd = 21.902r = -0.083 - r^2 = 0.007