Automated F(C)CS analysis of clone # 2148

Created on 12/08/2014
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Gene and protein information

Clone # 2148
Gene symbol: HP1BP3
Description: heterochromatin protein 1, binding protein 3
Molecular weight (with FP): 88.2 kDa
Localisation: granular nucleus, ave. nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2148_img_ch1.png2148_img_ch2.png


Point 1, intensity traces and correlation functions
2148_itr_1.png2148_cor_1.png

Point 2, intensity traces and correlation functions
2148_itr_2.png2148_cor_2.png

Point 3, intensity traces and correlation functions
2148_itr_3.png2148_cor_3.png

Point 4, intensity traces and correlation functions
2148_itr_4.png2148_cor_4.png

Point 5, intensity traces and correlation functions
2148_itr_5.png2148_cor_5.png

Point 6, intensity traces and correlation functions
2148_itr_6.png2148_cor_6.png

Sessions

1 20111117\U01--V01--2148
2 20111130\U02--V01--2148
3 20111201\U02--V01--2148
4 20120126\U02--V00--2148
5 20120215\U02--V00--2148
6 20120223\U01--V00--2148

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.310E+2 8.894E+1 9.353E+2 4.066E+2 1.672E+1 2.477E+1 3.793E+1 5.751E+1 5.128E+2 4.795E+2 6.755E+1 9.275E+1
N_corr 2.380E+2 1.698E+2 1.474E+3 7.571E+2 5.487E+1 7.074E+1 6.306E+1 1.314E+2 9.077E+2 9.930E+2 3.179E+2 4.657E+2
c [nM] 8.145E+2 5.531E+2 4.338E+3 1.886E+3 8.598E+1 1.274E+2 2.359E+2 3.577E+2 2.379E+3 2.224E+3 3.473E+2 4.768E+2
c_corr [nM] 1.480E+3 1.056E+3 6.838E+3 3.512E+3 2.821E+2 3.637E+2 3.922E+2 8.173E+2 4.210E+3 4.606E+3 1.634E+3 2.394E+3
ratioG 1.045E-1 7.818E-2 3.827E-1 2.672E-1
ratioG_corr 1.210E-1 1.237E-1 6.038E-1 5.109E-1
f_1 6.686E-1 1.263E-1 3.793E-1 2.205E-1 8.551E-1 1.735E-1 8.889E-1 1.018E-1 5.375E-1 3.622E-1 9.030E-1 1.712E-1
tauD_1 [ms] 3.158E+0 2.306E+0 1.860E+0 4.441E-16 1.045E+1 1.352E+1 8.535E-1 9.725E-1 1.860E+0 0.000E+0 2.055E+0 9.773E-1
D_1 [mum^2/s] 5.126E+0 3.592E+0 6.926E+0 0.000E+0 8.360E+0 2.181E+1 2.643E+1 2.359E+1 6.926E+0 0.000E+0 1.299E+1 2.112E+1
alpha_1 9.048E-1 1.050E-1 9.200E-1 1.110E-16 8.922E-1 2.258E-1 9.503E-1 1.570E-1 9.200E-1 0.000E+0 9.972E-1 2.635E-1
f_2 3.314E-1 1.263E-1 6.207E-1 2.205E-1 1.449E-1 1.735E-1 1.111E-1 1.018E-1 4.625E-1 3.622E-1 9.697E-2 1.712E-1
tauD_2 [ms] 5.910E+1 4.027E+1 8.505E+1 9.580E+1 3.471E+2 8.389E+2 2.081E+1 1.583E+1 6.048E+1 1.186E+2 8.495E+1 1.137E+2
D_2 [mum^2/s] 2.411E-1 1.184E-1 5.671E-1 7.685E-1 1.049E-1 7.534E-2 7.246E-1 3.900E-1 1.815E+0 1.813E+0 2.432E-1 9.562E-2
alpha_2 1.523E+0 3.088E-1 1.331E+0 3.452E-1 1.253E+0 2.979E-1 1.160E+0 2.137E-1 1.361E+0 4.383E-1 1.257E+0 3.523E-1
tauD_m [ms] 2.103E+1 1.314E+1 5.398E+1 6.915E+1 7.077E+1 1.793E+2 3.735E+0 6.374E+0 2.185E+1 5.356E+1 2.596E+1 6.410E+1
D_m [mum^2/s] 3.308E+0 1.940E+0 3.034E+0 1.342E+0 6.309E+0 1.601E+1 2.419E+1 2.230E+1 5.045E+0 1.396E+0 1.248E+1 2.127E+1
R [1/s] 3.812E+0 5.488E+0 3.342E+0 6.154E+0
CPM [kHz] 6.261E-1 2.346E-1 4.054E-1 3.488E-1 3.859E-1 2.747E-1 3.630E-1 4.446E-1
CPM_corr [kHz] 6.450E-1 2.396E-1 4.358E-1 3.746E-1 6.458E-1 4.479E-1 4.243E-1 4.760E-1
Int [kHz] 6.853E+1 3.375E+1 1.758E+2 4.196E+1 1.359E+1 2.122E+1 1.043E+2 7.085E+1
Int_corr [kHz] 1.263E+2 6.498E+1 2.919E+2 8.309E+1 2.395E+1 4.030E+1 1.922E+2 1.397E+2
# samples 176 176 124 124 90 90 88 88 16 16 11 11
# cells 65

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1480.297 - sd = 1056.174Int_corr [kHz] - Ch1 - Nuc : ave = 126.263 - sd = 64.975r = 0.735 - r^2 = 0.540
f_1 - Ch1 - Nuc : ave = 0.669 - sd = 0.126D_1 [mum^2/s] - Ch1 - Nuc : ave = 5.126 - sd = 3.592r = -0.518 - r^2 = 0.269
f_2 - Ch1 - Nuc : ave = 0.331 - sd = 0.126D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.241 - sd = 0.118r = 0.195 - r^2 = 0.038
CPM_corr [kHz] - Ch1 - Nuc : ave = 0.645 - sd = 0.240D_m [mum^2/s] - Ch1 - Nuc : ave = 3.308 - sd = 1.940r = -0.343 - r^2 = 0.117
c_corr [nM] - Ch1 - Nuc : ave = 1480.297 - sd = 1056.174ratioG_corr - Cross - Nuc : ave = 0.121 - sd = 0.124r = 0.390 - r^2 = 0.152
c_corr [nM] - Ch1 - Cyto : ave = 392.180 - sd = 817.285Int_corr [kHz] - Ch1 - Cyto : ave = 23.953 - sd = 40.297r = 0.701 - r^2 = 0.492
f_1 - Ch1 - Cyto : ave = 0.889 - sd = 0.102D_1 [mum^2/s] - Ch1 - Cyto : ave = 26.426 - sd = 23.589r = 0.146 - r^2 = 0.021
f_2 - Ch1 - Cyto : ave = 0.111 - sd = 0.102D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.725 - sd = 0.390r = -0.324 - r^2 = 0.105
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.646 - sd = 0.448D_m [mum^2/s] - Ch1 - Cyto : ave = 24.192 - sd = 22.301r = 0.093 - r^2 = 0.009