Automated F(C)CS analysis of clone # 2214

Created on 12/08/2014
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Gene and protein information

Clone # 2214
Gene symbol: NEIL2
Description: nei endonuclease VIII-like 2 (E. coli)
Molecular weight (with FP): 63.8 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2214_img_ch1.png2214_img_ch2.png


Point 1, intensity traces and correlation functions
2214_itr_1.png2214_cor_1.png

Point 2, intensity traces and correlation functions
2214_itr_2.png2214_cor_2.png

Point 3, intensity traces and correlation functions
2214_itr_3.png2214_cor_3.png

Point 4, intensity traces and correlation functions
2214_itr_4.png2214_cor_4.png

Point 5, intensity traces and correlation functions
2214_itr_5.png2214_cor_5.png

Point 6, intensity traces and correlation functions
2214_itr_6.png2214_cor_6.png

Sessions

1 20110915\U02--V01--2214
2 20110916\U02--V01--2214
3 20110920\U02--V01--2214

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 6.873E+1 4.972E+1 8.947E+2 4.404E+2 7.636E+0 9.979E+0 4.700E+1 4.503E+1 2.630E+2 2.550E+2 4.230E+1 4.217E+1
N_corr 7.678E+1 6.128E+1 1.349E+3 7.036E+2 9.645E+0 1.334E+1 5.363E+1 5.556E+1 2.619E+2 4.143E+2 5.368E+1 5.113E+1
c [nM] 4.274E+2 3.092E+2 4.150E+3 2.043E+3 3.926E+1 5.130E+1 2.923E+2 2.801E+2 1.220E+3 1.183E+3 2.175E+2 2.168E+2
c_corr [nM] 4.775E+2 3.811E+2 6.255E+3 3.263E+3 4.958E+1 6.856E+1 3.335E+2 3.456E+2 1.215E+3 1.922E+3 2.760E+2 2.628E+2
ratioG 7.965E-2 5.948E-2 5.740E-1 2.735E-1
ratioG_corr 9.084E-3 3.264E-2 1.067E-1 8.210E-2
f_1 9.008E-1 7.567E-2 4.165E-1 2.003E-1 9.961E-1 2.072E-2 9.285E-1 4.684E-2 8.349E-1 2.104E-1 9.735E-1 4.242E-2
tauD_1 [ms] 2.272E+0 7.211E-1 1.800E+0 0.000E+0 1.450E+0 1.230E+0 1.093E+0 3.659E-1 1.800E+0 0.000E+0 7.266E-1 4.241E-1
D_1 [mum^2/s] 5.196E+0 1.855E+0 7.157E+0 0.000E+0 1.682E+1 1.944E+1 1.052E+1 2.630E+0 7.157E+0 0.000E+0 2.009E+1 7.718E+0
alpha_1 1.005E+0 8.119E-2 9.200E-1 1.110E-16 7.937E-1 2.015E-1 9.337E-1 7.209E-2 9.252E-1 2.732E-2 7.880E-1 1.250E-1
f_2 9.923E-2 7.567E-2 5.835E-1 2.003E-1 3.893E-3 2.072E-2 7.146E-2 4.684E-2 1.651E-1 2.104E-1 2.651E-2 4.242E-2
tauD_2 [ms] 2.527E+1 1.824E+1 8.488E+1 1.015E+2 8.669E+1 5.555E+1 2.005E+1 9.840E+0 7.161E+1 9.383E+1 3.134E+1 3.164E+1
D_2 [mum^2/s] 5.478E-1 2.360E-1 5.724E-1 6.862E-1 2.010E-1 1.356E-1 6.144E-1 2.025E-1 4.747E-1 4.383E-1 5.975E-1 2.961E-1
alpha_2 1.356E+0 3.789E-1 1.309E+0 3.351E-1 1.088E+0 6.591E-2 1.030E+0 1.744E-1 1.207E+0 3.230E-1 1.138E+0 1.454E-1
tauD_m [ms] 4.468E+0 2.852E+0 4.852E+1 6.693E+1 1.655E+0 1.653E+0 2.621E+0 1.877E+0 2.159E+1 3.663E+1 1.313E+0 1.310E+0
D_m [mum^2/s] 4.702E+0 1.607E+0 3.394E+0 1.089E+0 1.679E+1 1.946E+1 9.795E+0 2.473E+0 6.058E+0 1.389E+0 1.955E+1 7.442E+0
R [1/s] 2.955E+1 2.660E+1 1.974E+1 2.328E+1
CPM [kHz] 1.517E+0 2.700E-1 3.150E-1 2.188E-1 1.390E+0 4.326E-1 1.879E-1 1.631E-1
CPM_corr [kHz] 1.567E+0 2.690E-1 3.566E-1 2.588E-1 1.470E+0 4.291E-1 6.630E-1 3.426E-1
Int [kHz] 1.171E+2 9.553E+1 1.507E+2 3.858E+1 7.741E+1 1.104E+2 4.649E+1 5.009E+1
Int_corr [kHz] 1.344E+2 1.296E+2 2.508E+2 7.422E+1 8.728E+1 1.188E+2 6.652E+1 7.990E+1
# samples 77 77 62 62 53 53 69 69 29 29 24 24
# cells 32

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 477.495 - sd = 381.114Int_corr [kHz] - Ch1 - Nuc : ave = 134.368 - sd = 129.623r = 0.971 - r^2 = 0.943
f_1 - Ch1 - Nuc : ave = 0.901 - sd = 0.076D_1 [mum^2/s] - Ch1 - Nuc : ave = 5.196 - sd = 1.855r = -0.203 - r^2 = 0.041
f_2 - Ch1 - Nuc : ave = 0.099 - sd = 0.076D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.548 - sd = 0.236r = 0.011 - r^2 = 0.000
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.567 - sd = 0.269D_m [mum^2/s] - Ch1 - Nuc : ave = 4.702 - sd = 1.607r = 0.161 - r^2 = 0.026
c_corr [nM] - Ch1 - Nuc : ave = 477.495 - sd = 381.114ratioG_corr - Cross - Nuc : ave = 0.009 - sd = 0.033r = 0.375 - r^2 = 0.141
c_corr [nM] - Ch1 - Cyto : ave = 333.543 - sd = 345.568Int_corr [kHz] - Ch1 - Cyto : ave = 87.284 - sd = 118.812r = 0.939 - r^2 = 0.882
f_1 - Ch1 - Cyto : ave = 0.929 - sd = 0.047D_1 [mum^2/s] - Ch1 - Cyto : ave = 10.517 - sd = 2.630r = 0.173 - r^2 = 0.030
f_2 - Ch1 - Cyto : ave = 0.071 - sd = 0.047D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.614 - sd = 0.202r = -0.305 - r^2 = 0.093
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.470 - sd = 0.429D_m [mum^2/s] - Ch1 - Cyto : ave = 9.795 - sd = 2.473r = 0.208 - r^2 = 0.043