Automated F(C)CS analysis of clone # 2444

Created on 12/08/2014
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Gene and protein information

Clone # 2444
Gene symbol: RFX7
Description: regulatory factor X, 7
Molecular weight (with FP): 184.3 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2444_img_ch1.png2444_img_ch2.png


Point 1, intensity traces and correlation functions
2444_itr_1.png2444_cor_1.png

Point 2, intensity traces and correlation functions
2444_itr_2.png2444_cor_2.png

Point 3, intensity traces and correlation functions
2444_itr_3.png2444_cor_3.png

Point 4, intensity traces and correlation functions
2444_itr_4.png2444_cor_4.png

Point 5, intensity traces and correlation functions
2444_itr_5.png2444_cor_5.png

Point 6, intensity traces and correlation functions
2444_itr_6.png2444_cor_6.png

Sessions

1 20111117\U03--V01--2444
2 20111129\U03--V01--2444
3 20111130\U03--V01--2444
4 20111201\U03--V01--2444
5 20111206\U03--V01--2444
6 20120127\U02--V00--2444

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 5.784E+1 4.195E+1 8.138E+2 4.280E+2 4.436E+0 4.641E+0 7.903E+1 6.161E+1 1.638E+2 1.692E+2 4.022E+1 4.343E+1
N_corr 6.517E+1 6.510E+1 1.287E+3 8.101E+2 5.573E+0 7.486E+0 1.153E+2 1.030E+2 1.415E+2 3.188E+2 7.565E+1 8.136E+1
c [nM] 3.597E+2 2.609E+2 3.775E+3 1.985E+3 2.280E+1 2.386E+1 4.915E+2 3.832E+2 7.596E+2 7.847E+2 2.068E+2 2.233E+2
c_corr [nM] 4.053E+2 4.049E+2 5.969E+3 3.757E+3 2.865E+1 3.849E+1 7.169E+2 6.409E+2 6.563E+2 1.479E+3 3.889E+2 4.183E+2
ratioG 5.903E-2 3.594E-2 5.762E-1 2.368E-1
ratioG_corr 1.328E-2 4.315E-2 1.357E-1 1.400E-1
f_1 9.145E-1 9.567E-2 4.344E-1 2.484E-1 9.858E-1 4.874E-2 7.671E-1 1.338E-1 5.584E-1 2.991E-1 8.538E-1 1.428E-1
tauD_1 [ms] 6.949E-1 1.049E+0 1.860E+0 4.441E-16 4.672E-1 6.246E-1 2.075E+0 2.432E+0 1.860E+0 2.220E-16 1.598E+0 2.167E+0
D_1 [mum^2/s] 3.318E+1 1.768E+1 6.926E+0 2.665E-15 6.278E+1 4.217E+1 1.560E+1 1.568E+1 6.926E+0 0.000E+0 2.664E+1 3.044E+1
alpha_1 1.046E+0 9.302E-2 9.200E-1 6.661E-16 1.039E+0 2.029E-1 9.707E-1 1.487E-1 9.200E-1 1.110E-16 7.286E-1 2.319E-1
f_2 8.549E-2 9.567E-2 5.656E-1 2.484E-1 1.425E-2 4.874E-2 2.329E-1 1.338E-1 4.416E-1 2.991E-1 1.462E-1 1.428E-1
tauD_2 [ms] 1.658E+1 4.449E+1 7.788E+1 1.101E+2 5.053E+1 2.622E+1 6.242E+1 7.514E+1 4.338E+1 5.617E+1 6.447E+1 5.197E+1
D_2 [mum^2/s] 1.627E+0 8.773E-1 7.577E-1 1.026E+0 2.971E-1 1.416E-1 3.766E-1 3.306E-1 7.154E-1 7.614E-1 2.980E-1 2.367E-1
alpha_2 1.224E+0 2.438E-1 1.414E+0 3.872E-1 1.106E+0 1.436E-1 1.351E+0 3.491E-1 1.151E+0 1.957E-1 1.377E+0 3.690E-1
tauD_m [ms] 3.911E+0 1.816E+1 3.946E+1 6.052E+1 1.095E+0 2.500E+0 1.795E+1 2.468E+1 1.776E+1 1.930E+1 1.003E+1 9.501E+0
D_m [mum^2/s] 3.145E+1 1.760E+1 3.588E+0 1.306E+0 6.214E+1 4.198E+1 1.273E+1 1.371E+1 4.217E+0 1.791E+0 2.251E+1 2.705E+1
R [1/s] 1.817E+2 1.230E+2 1.253E+1 4.201E+1
CPM [kHz] 1.105E+0 4.537E-1 4.529E-1 3.201E-1 1.307E+0 7.267E-1 2.415E-1 2.204E-1
CPM_corr [kHz] 1.149E+0 4.544E-1 4.804E-1 3.329E-1 1.348E+0 7.412E-1 3.570E-1 5.116E+0
Int [kHz] 7.237E+1 5.794E+1 2.063E+2 7.911E+1 8.393E+1 6.440E+1 3.391E+1 4.132E+1
Int_corr [kHz] 8.025E+1 6.298E+1 3.361E+2 1.464E+2 1.193E+2 9.303E+1 5.455E+1 8.407E+1
# samples 270 270 247 247 95 95 257 257 53 53 48 48
# cells 114

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 405.294 - sd = 404.871Int_corr [kHz] - Ch1 - Nuc : ave = 80.247 - sd = 62.979r = 0.662 - r^2 = 0.439
f_1 - Ch1 - Nuc : ave = 0.915 - sd = 0.096D_1 [mum^2/s] - Ch1 - Nuc : ave = 33.181 - sd = 17.680r = 0.546 - r^2 = 0.298
f_2 - Ch1 - Nuc : ave = 0.085 - sd = 0.096D_2 [mum^2/s] - Ch1 - Nuc : ave = 1.627 - sd = 0.877r = -0.465 - r^2 = 0.216
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.149 - sd = 0.454D_m [mum^2/s] - Ch1 - Nuc : ave = 31.455 - sd = 17.599r = 0.247 - r^2 = 0.061
c_corr [nM] - Ch1 - Nuc : ave = 405.294 - sd = 404.871ratioG_corr - Cross - Nuc : ave = 0.013 - sd = 0.043r = -0.139 - r^2 = 0.019
c_corr [nM] - Ch1 - Cyto : ave = 716.893 - sd = 640.880Int_corr [kHz] - Ch1 - Cyto : ave = 119.323 - sd = 93.031r = 0.707 - r^2 = 0.499
f_1 - Ch1 - Cyto : ave = 0.767 - sd = 0.134D_1 [mum^2/s] - Ch1 - Cyto : ave = 15.604 - sd = 15.677r = 0.243 - r^2 = 0.059
f_2 - Ch1 - Cyto : ave = 0.233 - sd = 0.134D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.377 - sd = 0.331r = -0.396 - r^2 = 0.157
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.348 - sd = 0.741D_m [mum^2/s] - Ch1 - Cyto : ave = 12.727 - sd = 13.709r = 0.004 - r^2 = 1.633E-5