Automated F(C)CS analysis of clone # 2542

Created on 12/08/2014
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Gene and protein information

Clone # 2542
Gene symbol: USP37
Description: ubiquitin specific peptidase 37
Molecular weight (with FP): 137.2 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2542_img_ch1.png2542_img_ch2.png


Point 1, intensity traces and correlation functions
2542_itr_1.png2542_cor_1.png

Point 2, intensity traces and correlation functions
2542_itr_2.png2542_cor_2.png

Point 3, intensity traces and correlation functions
2542_itr_3.png2542_cor_3.png

Point 4, intensity traces and correlation functions
2542_itr_4.png2542_cor_4.png

Point 5, intensity traces and correlation functions
2542_itr_5.png2542_cor_5.png

Point 6, intensity traces and correlation functions
2542_itr_6.png2542_cor_6.png

Sessions

1 20111123\U03--V01--2542
2 20111125\U01--V01--2542
3 20111207\U01--V00--2542
4 20120126\U01--V00--2542

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.363E+2 9.966E+1 1.033E+3 4.999E+2 2.141E+1 2.663E+1 2.956E+1 2.002E+1 5.342E+2 3.466E+2 6.705E+0 7.395E+0
N_corr 1.539E+2 1.240E+2 1.424E+3 7.989E+2 4.115E+1 1.176E+2 3.229E+1 2.428E+1 8.638E+2 6.148E+2 1.076E+1 1.561E+1
c [nM] 8.478E+2 6.198E+2 4.791E+3 2.319E+3 1.101E+2 1.369E+2 1.838E+2 1.245E+2 2.478E+3 1.608E+3 3.447E+1 3.802E+1
c_corr [nM] 9.574E+2 7.715E+2 6.607E+3 3.706E+3 2.116E+2 6.047E+2 2.008E+2 1.510E+2 4.007E+3 2.852E+3 5.533E+1 8.025E+1
ratioG 1.113E-1 8.132E-2 1.058E-1 8.518E-2
ratioG_corr 4.401E-2 1.603E-1 7.233E-2 1.034E-1
f_1 8.644E-1 1.731E-1 3.764E-1 2.069E-1 9.799E-1 5.398E-2 8.853E-1 1.549E-1 5.756E-1 2.994E-1 9.445E-1 1.081E-1
tauD_1 [ms] 1.450E+0 1.164E+0 1.860E+0 4.441E-16 1.068E+0 9.488E-1 5.912E-1 8.077E-1 1.860E+0 4.441E-16 1.534E+0 8.413E-1
D_1 [mum^2/s] 1.240E+1 9.551E+0 6.926E+0 0.000E+0 2.327E+1 2.162E+1 2.829E+1 1.227E+1 6.926E+0 0.000E+0 2.160E+1 3.521E+1
alpha_1 9.919E-1 9.598E-2 9.200E-1 1.110E-16 8.500E-1 2.314E-1 1.037E+0 9.239E-2 9.200E-1 0.000E+0 9.994E-1 2.354E-1
f_2 1.356E-1 1.731E-1 6.236E-1 2.069E-1 2.006E-2 5.398E-2 1.147E-1 1.549E-1 4.244E-1 2.994E-1 5.555E-2 1.081E-1
tauD_2 [ms] 1.896E+1 1.133E+1 5.172E+1 7.131E+1 6.797E+1 3.865E+1 1.876E+1 2.145E+1 5.601E+1 5.842E+1 8.389E+1 4.778E+1
D_2 [mum^2/s] 9.587E-1 1.821E+0 7.284E-1 8.973E-1 2.274E-1 1.498E-1 1.433E+0 2.955E+0 6.293E-1 8.385E-1 2.126E-1 1.597E-1
alpha_2 1.272E+0 2.892E-1 1.375E+0 3.422E-1 1.099E+0 7.066E-2 1.055E+0 2.009E-1 1.395E+0 4.092E-1 1.120E+0 3.405E-1
tauD_m [ms] 3.626E+0 3.458E+0 3.047E+1 4.207E+1 3.293E+0 9.640E+0 2.693E+0 4.008E+0 2.148E+1 2.573E+1 8.096E+0 1.450E+1
D_m [mum^2/s] 1.117E+1 8.706E+0 3.161E+0 1.125E+0 2.284E+1 2.138E+1 2.640E+1 1.119E+1 4.415E+0 1.601E+0 2.089E+1 3.538E+1
R [1/s] 7.115E+1 2.716E+2 7.273E+1 4.481E+1
CPM [kHz] 1.196E+0 2.848E-1 3.502E-1 2.599E-1 9.613E-1 3.634E-1 3.291E-1 1.679E-1
CPM_corr [kHz] 1.216E+0 2.856E-1 4.039E-1 3.017E-1 1.086E+0 3.422E-1 3.651E-1 1.753E-1
Int [kHz] 1.719E+2 1.231E+2 2.039E+2 6.080E+1 3.428E+1 3.008E+1 1.362E+2 7.780E+1
Int_corr [kHz] 1.893E+2 1.325E+2 3.202E+2 1.141E+2 3.978E+1 3.409E+1 2.347E+2 1.313E+2
# samples 144 144 124 124 98 98 117 117 12 12 9 9
# cells 56

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 957.450 - sd = 771.488Int_corr [kHz] - Ch1 - Nuc : ave = 189.274 - sd = 132.463r = 0.855 - r^2 = 0.730
f_1 - Ch1 - Nuc : ave = 0.864 - sd = 0.173D_1 [mum^2/s] - Ch1 - Nuc : ave = 12.396 - sd = 9.551r = -0.254 - r^2 = 0.064
f_2 - Ch1 - Nuc : ave = 0.136 - sd = 0.173D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.959 - sd = 1.821r = 0.239 - r^2 = 0.057
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.216 - sd = 0.286D_m [mum^2/s] - Ch1 - Nuc : ave = 11.172 - sd = 8.706r = -0.047 - r^2 = 0.002
c_corr [nM] - Ch1 - Nuc : ave = 957.450 - sd = 771.488ratioG_corr - Cross - Nuc : ave = 0.044 - sd = 0.160r = 0.090 - r^2 = 0.008
c_corr [nM] - Ch1 - Cyto : ave = 200.799 - sd = 150.976Int_corr [kHz] - Ch1 - Cyto : ave = 39.781 - sd = 34.085r = 0.882 - r^2 = 0.779
f_1 - Ch1 - Cyto : ave = 0.885 - sd = 0.155D_1 [mum^2/s] - Ch1 - Cyto : ave = 28.287 - sd = 12.269r = 0.561 - r^2 = 0.314
f_2 - Ch1 - Cyto : ave = 0.115 - sd = 0.155D_2 [mum^2/s] - Ch1 - Cyto : ave = 1.433 - sd = 2.955r = -0.374 - r^2 = 0.140
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.086 - sd = 0.342D_m [mum^2/s] - Ch1 - Cyto : ave = 26.400 - sd = 11.186r = 0.186 - r^2 = 0.035