Automated F(C)CS analysis of clone # 2672

Created on 12/08/2014
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Gene and protein information

Clone # 2672
Gene symbol: UTP15
Description: UTP15, U3 small nucleolar ribonucleoprotein, homolog (S. cerevisiae)
Molecular weight (with FP): 85.4 kDa
Localisation: homogeneous nucleus, bright nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
2672_img_ch1.png2672_img_ch2.png


Point 1, intensity traces and correlation functions
2672_itr_1.png2672_cor_1.png

Point 2, intensity traces and correlation functions
2672_itr_2.png2672_cor_2.png

Point 3, intensity traces and correlation functions
2672_itr_3.png2672_cor_3.png

Point 4, intensity traces and correlation functions
2672_itr_4.png2672_cor_4.png

Point 5, intensity traces and correlation functions
2672_itr_5.png2672_cor_5.png

Point 6, intensity traces and correlation functions
2672_itr_6.png2672_cor_6.png

Sessions

1 20111123\U02--V01--2672
2 20111125\U00--V01--2672
3 20120307\U01--V00--2672
4 20120315\U02--V00--2672

Results table

Ch1 ave
Nuc
Ch1 sd
Nuc
Ch2 ave
Nuc
Ch2 sd
Nuc
Cross ave
Nuc
Cross sd
Nuc
Ch1 ave
Cyto
Ch1 sd
Cyto
Ch2 ave
Cyto
Ch2 sd
Cyto
Cross ave
Cyto
Cross sd
Cyto
N 5.146E+1 2.176E+1 1.096E+3 5.643E+2 2.829E+0 2.389E+0 5.560E+1 2.692E+1 1.581E+2 1.699E+2 3.764E+1 2.383E+1
N_corr 5.966E+1 3.029E+1 1.855E+3 1.048E+3 3.361E+0 3.032E+0 6.256E+1 3.449E+1 1.176E+2 3.056E+2 4.929E+1 4.409E+1
c [nM] 3.201E+2 1.353E+2 5.082E+3 2.617E+3 1.455E+1 1.228E+1 3.458E+2 1.674E+2 7.331E+2 7.883E+2 1.935E+2 1.225E+2
c_corr [nM] 3.711E+2 1.884E+2 8.603E+3 4.863E+3 1.728E+1 1.559E+1 3.891E+2 2.145E+2 5.454E+2 1.418E+3 2.534E+2 2.267E+2
ratioG 4.753E-2 2.543E-2 6.168E-1 1.996E-1
ratioG_corr 6.150E-3 1.775E-2 1.226E-1 1.003E-1
f_1 8.982E-1 5.114E-2 3.600E-1 2.191E-1 9.886E-1 3.580E-2 8.883E-1 5.561E-2 9.202E-1 1.623E-1 9.793E-1 4.908E-2
tauD_1 [ms] 5.366E-1 2.921E-1 1.860E+0 2.220E-16 4.643E-1 5.741E-1 9.225E-1 4.915E-1 1.860E+0 4.441E-16 5.555E-1 3.255E-1
D_1 [mum^2/s] 2.375E+1 8.672E+0 6.926E+0 1.776E-15 5.447E+1 3.706E+1 1.448E+1 6.406E+0 6.926E+0 8.882E-16 2.947E+1 1.565E+1
alpha_1 1.021E+0 8.100E-2 9.200E-1 0.000E+0 1.025E+0 2.145E-1 1.003E+0 9.110E-2 9.200E-1 0.000E+0 7.725E-1 1.544E-1
f_2 1.018E-1 5.114E-2 6.400E-1 2.191E-1 1.141E-2 3.580E-2 1.117E-1 5.561E-2 7.980E-2 1.623E-1 2.072E-2 4.908E-2
tauD_2 [ms] 1.163E+1 6.493E+0 7.174E+1 1.047E+2 6.134E+1 3.756E+1 2.373E+1 1.902E+1 4.885E+1 8.963E+1 2.720E+1 2.408E+1
D_2 [mum^2/s] 1.151E+0 4.993E-1 6.994E-1 8.551E-1 2.658E-1 1.645E-1 6.259E-1 3.127E-1 6.020E-1 4.933E-1 6.372E-1 3.091E-1
alpha_2 1.189E+0 1.733E-1 1.316E+0 3.189E-1 1.078E+0 5.817E-2 1.105E+0 1.592E-1 1.097E+0 1.382E-1 1.103E+0 1.131E-1
tauD_m [ms] 1.819E+0 1.407E+0 4.387E+1 6.871E+1 1.245E+0 2.644E+0 3.712E+0 3.822E+0 6.757E+0 1.478E+1 1.238E+0 3.258E+0
D_m [mum^2/s] 2.161E+1 8.453E+0 3.032E+0 1.227E+0 5.402E+1 3.717E+1 1.284E+1 5.638E+0 6.458E+0 8.547E-1 2.876E+1 1.510E+1
R [1/s] 1.124E+2 7.171E+1 2.256E+1 3.374E+1
CPM [kHz] 1.246E+0 2.354E-1 3.289E-1 3.753E-1 1.487E+0 5.026E-1 1.637E-1 1.946E-1
CPM_corr [kHz] 1.285E+0 2.377E-1 3.474E-1 3.899E-1 1.529E+0 5.125E-1 7.732E-1 5.017E-1
Int [kHz] 7.387E+1 3.281E+1 2.019E+2 5.888E+1 8.740E+1 4.843E+1 2.408E+1 2.532E+1
Int_corr [kHz] 8.579E+1 3.807E+1 3.593E+2 1.272E+2 9.782E+1 5.159E+1 3.597E+1 6.233E+1
# samples 198 198 149 149 86 86 172 172 86 86 81 81
# cells 67

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 371.063 - sd = 188.360Int_corr [kHz] - Ch1 - Nuc : ave = 85.787 - sd = 38.074r = 0.858 - r^2 = 0.737
f_1 - Ch1 - Nuc : ave = 0.898 - sd = 0.051D_1 [mum^2/s] - Ch1 - Nuc : ave = 23.755 - sd = 8.672r = 0.612 - r^2 = 0.375
f_2 - Ch1 - Nuc : ave = 0.102 - sd = 0.051D_2 [mum^2/s] - Ch1 - Nuc : ave = 1.151 - sd = 0.499r = -0.717 - r^2 = 0.514
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.285 - sd = 0.238D_m [mum^2/s] - Ch1 - Nuc : ave = 21.612 - sd = 8.453r = 0.284 - r^2 = 0.081
c_corr [nM] - Ch1 - Nuc : ave = 371.063 - sd = 188.360ratioG_corr - Cross - Nuc : ave = 0.006 - sd = 0.018r = 0.317 - r^2 = 0.100
c_corr [nM] - Ch1 - Cyto : ave = 389.071 - sd = 214.515Int_corr [kHz] - Ch1 - Cyto : ave = 97.822 - sd = 51.593r = 0.643 - r^2 = 0.413
f_1 - Ch1 - Cyto : ave = 0.888 - sd = 0.056D_1 [mum^2/s] - Ch1 - Cyto : ave = 14.481 - sd = 6.406r = -0.308 - r^2 = 0.095
f_2 - Ch1 - Cyto : ave = 0.112 - sd = 0.056D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.626 - sd = 0.313r = -0.029 - r^2 = 0.001
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.529 - sd = 0.513D_m [mum^2/s] - Ch1 - Cyto : ave = 12.844 - sd = 5.638r = 0.152 - r^2 = 0.023