Automated F(C)CS analysis of clone # EYFPC1

Created on 12/08/2014
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Gene and protein information

Clone # EYFPC1
Gene symbol: EYFPC1
Description: enhanced yellow fluorescent protein C1
Molecular weight (with FP): 27.0 kDa
Localisation: homogeneous nucleus, ave. nucleoli, no speckles, cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
EYFPC1_img_ch1.pngEYFPC1_img_ch2.png


Point 1, intensity traces and correlation functions
EYFPC1_itr_1.pngEYFPC1_cor_1.png

Point 2, intensity traces and correlation functions
EYFPC1_itr_2.pngEYFPC1_cor_2.png

Point 3, intensity traces and correlation functions
EYFPC1_itr_3.pngEYFPC1_cor_3.png

Point 4, intensity traces and correlation functions
EYFPC1_itr_4.pngEYFPC1_cor_4.png

Point 5, intensity traces and correlation functions
EYFPC1_itr_5.pngEYFPC1_cor_5.png

Point 6, intensity traces and correlation functions
EYFPC1_itr_6.pngEYFPC1_cor_6.png

Sessions

1 20110826\U00--V00--EYFPC1
2 20110826\U01--V00--EYFPC1
3 20110831\U00--V00--EYFPC1
4 20110901\U00--V00--EYFPC1
5 20110902\U00--V00--EYFPC1
6 20110906\U00--V00--EYFPC1
7 20110907\U00--V00--EYFPC1
8 20110908\U00--V00--EYFPC1
9 20110909\U00--V00--EYFPC1
10 20110913\U00--V00--EYFPC1
11 20110914\U00--V00--EYFPC1
12 20110915\U00--V00--EYFPC1
13 20110916\U00--V00--EYFPC1
14 20110920\U00--V00--EYFPC1
15 20110921\U01--V00--EYFPC1
16 20110922\U00--V00--EYFPC1
17 20110927\U00--V00--EYFPC1
18 20110930\U00--V00--EYFPC1
19 20111011\U00--V00--EYFPC1
20 20111012\U00--V00--EYFPC1
21 20111013\U00--V00--EYFPC1
22 20111104\U00--V00--EYFPC1
23 20111108\U00--V00--EYFPC1
24 20111109\U00--V00--EYFPC1
25 20111110\U00--V00--EYFPC1
26 20111117\U00--V00--EYFPC1
27 20111123\U00--V00--EYFPC1
28 20111125\U00--V00--EYFPC1
29 20111129\U00--V00--EYFPC1
30 20111130\U00--V00--EYFPC1
31 20111201\U00--V00--EYFPC1
32 20111208\U00--V00--EYFPC1
33 20111209\U00--V00--EYFPC1
34 20111213\U00--V00--EYFPC1
35 20111215\U00--V00--EYFPC1
36 20111216\U00--V00--EYFPC1
37 20120116\U00--V00--EYFPC1
38 20120117\U00--V00--EYFPC1
39 20120712\U02--V00--EYFPC1
40 20120712\U02--V01--EYFPC1

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 8.844E+1 7.861E+1 1.341E+3 7.190E+2 8.676E+0 1.241E+1 6.719E+1 7.953E+1 3.005E+2 3.395E+2 3.523E+1 3.424E+1
N_corr 9.027E+1 8.061E+1 2.053E+3 1.281E+3 9.147E+0 1.314E+1 7.136E+1 8.391E+1 3.680E+2 6.345E+2 3.984E+1 3.720E+1
c [nM] 5.500E+2 4.889E+2 6.221E+3 3.335E+3 4.460E+1 6.382E+1 4.179E+2 4.946E+2 1.394E+3 1.575E+3 1.811E+2 1.760E+2
c_corr [nM] 5.614E+2 5.014E+2 9.521E+3 5.940E+3 4.702E+1 6.754E+1 4.438E+2 5.219E+2 1.707E+3 2.943E+3 2.048E+2 1.912E+2
ratioG 7.590E-2 4.725E-2 4.745E-1 3.030E-1
ratioG_corr 1.335E-2 2.769E-2 8.646E-2 8.600E-2
f_1 9.744E-1 3.898E-2 4.521E-1 2.554E-1 9.919E-1 2.815E-2 9.522E-1 6.080E-2 8.806E-1 1.942E-1 9.712E-1 3.915E-2
tauD_1 [ms] 3.909E-1 1.563E-1 1.860E+0 4.219E-15 3.233E-1 3.181E-1 4.699E-1 8.987E-1 1.860E+0 2.220E-16 2.895E-1 2.183E-1
D_1 [mum^2/s] 3.015E+1 8.618E+0 6.926E+0 7.105E-15 8.207E+1 1.409E+2 2.962E+1 9.893E+0 6.926E+0 3.553E-15 5.744E+1 7.582E+1
alpha_1 1.035E+0 8.252E-2 9.200E-1 1.332E-15 1.052E+0 2.536E-1 1.026E+0 1.203E-1 9.200E-1 8.882E-16 1.002E+0 2.185E-1
f_2 2.561E-2 3.898E-2 5.479E-1 2.554E-1 8.143E-3 2.815E-2 4.776E-2 6.080E-2 1.194E-1 1.942E-1 2.881E-2 3.915E-2
tauD_2 [ms] 1.499E+1 2.060E+1 8.102E+1 9.726E+1 3.953E+1 3.215E+1 1.601E+1 1.900E+1 4.824E+1 8.288E+1 2.143E+1 2.739E+1
D_2 [mum^2/s] 1.533E+0 8.091E-1 5.657E-1 7.518E-1 4.763E-1 7.388E-1 1.237E+0 6.703E-1 1.224E+0 2.664E+0 1.002E+0 6.349E-1
alpha_2 1.108E+0 8.294E-2 1.274E+0 3.066E-1 1.077E+0 4.929E-2 1.080E+0 1.065E-1 1.143E+0 2.644E-1 1.076E+0 7.883E-2
tauD_m [ms] 6.187E-1 4.393E-1 4.644E+1 6.692E+1 7.201E-1 2.587E+0 1.213E+0 2.263E+0 1.328E+1 4.244E+1 1.012E+0 4.976E+0
D_m [mum^2/s] 2.942E+1 8.469E+0 3.494E+0 1.561E+0 8.155E+1 1.407E+2 2.826E+1 9.558E+0 6.340E+0 1.228E+0 5.576E+1 7.570E+1
R [1/s] 1.627E+2 1.181E+2 9.424E+1 8.973E+1
CPM [kHz] 1.122E+0 1.935E-1 3.389E-1 3.365E-1 1.086E+0 3.627E-1 2.139E-1 2.225E-1
CPM_corr [kHz] 1.150E+0 1.952E-1 3.721E-1 3.670E-1 1.128E+0 3.618E-1 6.648E-1 4.676E-1
Int [kHz] 1.152E+2 9.514E+1 2.076E+2 8.890E+1 8.101E+1 9.434E+1 5.461E+1 5.971E+1
Int_corr [kHz] 1.196E+2 9.839E+1 3.413E+2 1.608E+2 8.661E+1 9.584E+1 8.448E+1 1.158E+2
# samples 1545 1545 849 849 580 580 1752 1752 289 289 251 251
# cells 743

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 561.430 - sd = 501.355Int_corr [kHz] - Ch1 - Nuc : ave = 119.559 - sd = 98.393r = 0.922 - r^2 = 0.850
f_1 - Ch1 - Nuc : ave = 0.974 - sd = 0.039D_1 [mum^2/s] - Ch1 - Nuc : ave = 30.147 - sd = 8.618r = -0.083 - r^2 = 0.007
f_2 - Ch1 - Nuc : ave = 0.026 - sd = 0.039D_2 [mum^2/s] - Ch1 - Nuc : ave = 1.533 - sd = 0.809r = -0.044 - r^2 = 0.002
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.150 - sd = 0.195D_m [mum^2/s] - Ch1 - Nuc : ave = 29.422 - sd = 8.469r = 0.080 - r^2 = 0.006
c_corr [nM] - Ch1 - Nuc : ave = 561.430 - sd = 501.355ratioG_corr - Cross - Nuc : ave = 0.013 - sd = 0.028r = 0.041 - r^2 = 0.002
c_corr [nM] - Ch1 - Cyto : ave = 443.807 - sd = 521.875Int_corr [kHz] - Ch1 - Cyto : ave = 86.613 - sd = 95.844r = 0.804 - r^2 = 0.647
f_1 - Ch1 - Cyto : ave = 0.952 - sd = 0.061D_1 [mum^2/s] - Ch1 - Cyto : ave = 29.620 - sd = 9.893r = -0.009 - r^2 = 8.803E-5
f_2 - Ch1 - Cyto : ave = 0.048 - sd = 0.061D_2 [mum^2/s] - Ch1 - Cyto : ave = 1.237 - sd = 0.670r = -0.087 - r^2 = 0.008
CPM_corr [kHz] - Ch1 - Cyto : ave = 1.128 - sd = 0.362D_m [mum^2/s] - Ch1 - Cyto : ave = 28.259 - sd = 9.558r = 0.280 - r^2 = 0.079