Automated F(C)CS analysis of clone # HP1hi

Created on 12/08/2014
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Gene and protein information

Clone # HP1hi
Gene symbol: CBX5
Description: chromobox homolog 5, heterochromatin protein 1 isoform alpha
Molecular weight (with FP): 49.2 kDa
Localisation: granular nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
HP1hi_img_ch1.pngHP1hi_img_ch2.png


Point 1, intensity traces and correlation functions
HP1hi_itr_1.pngHP1hi_cor_1.png

Point 2, intensity traces and correlation functions
HP1hi_itr_2.pngHP1hi_cor_2.png

Point 3, intensity traces and correlation functions
HP1hi_itr_3.pngHP1hi_cor_3.png

Point 4, intensity traces and correlation functions
HP1hi_itr_4.pngHP1hi_cor_4.png

Point 5, intensity traces and correlation functions
HP1hi_itr_5.pngHP1hi_cor_5.png

Point 6, intensity traces and correlation functions
HP1hi_itr_6.pngHP1hi_cor_6.png

Sessions

1 20130109\U01--V00--HP1hi
2 20130110\U00--V01--HP1hi
3 20130117\X00--Y00--HP1hi
4 20130118\X00--Y00--HP1hi
5 20130118\X01--Y00--HP1hi
6 20130118\X02--Y00--HP1hi
7 20130118\X03--Y00--HP1hi
8 20130125\U01--V00--HP1hi
9 20130125\U01--V01--HP1hi
10 20130129\X00--Y00--HP1hi
11 20130129\X01--Y00--HP1hi
12 20130129\X02--Y00--HP1hi
13 20130129\X03--Y00--HP1hi
14 20130129\X05--Y00--HP1hi
15 20130130\X00--Y00--HP1hi
16 20130130\X01--Y00--HP1hi
17 20130130\X03--Y00--HP1hi
18 20130131\U00--V01--HP1hi

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 5.973E+2 3.563E+2 1.531E+3 7.982E+2 1.339E+2 1.043E+2 7.103E+1 1.130E+2 4.091E+2 2.769E+2 6.911E+1 4.669E+1
N_corr 6.016E+2 3.604E+2 1.617E+3 8.803E+2 1.425E+2 1.152E+2 6.843E+1 1.181E+2 4.368E+2 3.124E+2 7.791E+1 5.712E+1
c [nM] 3.715E+3 2.216E+3 7.101E+3 3.702E+3 6.882E+2 5.364E+2 4.418E+2 7.029E+2 1.898E+3 1.284E+3 3.553E+2 2.400E+2
c_corr [nM] 3.741E+3 2.241E+3 7.501E+3 4.083E+3 7.328E+2 5.920E+2 4.256E+2 7.343E+2 2.026E+3 1.449E+3 4.005E+2 2.936E+2
ratioG 2.479E-1 1.148E-1 3.881E-1 1.633E-1
ratioG_corr 1.334E-1 1.233E-1 2.771E-1 1.871E-1
f_1 8.593E-1 1.788E-1 2.601E-1 1.948E-1 7.723E-1 2.029E-1 8.711E-1 1.282E-1 5.188E-1 2.246E-1 7.278E-1 1.605E-1
tauD_1 [ms] 6.823E+0 4.343E+0 1.860E+0 8.882E-16 5.645E+0 9.893E+0 1.204E+0 1.471E+0 1.860E+0 0.000E+0 3.395E+0 4.437E+0
D_1 [mum^2/s] 2.470E+0 4.369E+0 6.926E+0 6.217E-15 9.342E+0 1.756E+1 2.601E+1 2.487E+1 6.926E+0 0.000E+0 6.202E+0 3.615E+0
alpha_1 9.714E-1 1.300E-1 9.232E-1 2.378E-2 9.340E-1 2.376E-1 9.929E-1 1.926E-1 9.200E-1 0.000E+0 9.976E-1 1.813E-1
f_2 1.407E-1 1.788E-1 7.399E-1 1.948E-1 2.277E-1 2.029E-1 1.289E-1 1.282E-1 4.812E-1 2.246E-1 2.722E-1 1.605E-1
tauD_2 [ms] 5.897E+1 3.106E+1 6.723E+1 3.300E+1 1.195E+2 7.564E+1 3.427E+1 4.643E+1 1.125E+2 7.318E+1 1.025E+2 7.603E+1
D_2 [mum^2/s] 2.174E-1 9.775E-2 2.529E-1 2.140E-1 1.366E-1 7.491E-2 6.722E-1 4.278E-1 1.821E-1 1.395E-1 1.621E-1 7.122E-2
alpha_2 1.272E+0 2.858E-1 1.432E+0 3.187E-1 1.473E+0 3.995E-1 1.191E+0 2.605E-1 1.616E+0 3.615E-1 1.578E+0 4.038E-1
tauD_m [ms] 1.433E+1 1.122E+1 4.846E+1 2.419E+1 3.562E+1 4.425E+1 8.463E+0 1.856E+1 5.039E+1 4.044E+1 3.634E+1 4.272E+1
D_m [mum^2/s] 1.937E+0 2.887E+0 1.994E+0 1.285E+0 6.689E+0 1.166E+1 2.393E+1 2.443E+1 3.699E+0 1.458E+0 4.320E+0 2.241E+0
R [1/s] 5.590E+0 1.239E+1 3.976E+0 6.206E+0
CPM [kHz] 4.720E-1 2.560E-1 3.367E-1 1.796E-1 4.204E-1 4.053E-1 4.867E-1 5.244E-1
CPM_corr [kHz] 4.776E-1 2.549E-1 3.866E-1 2.137E-1 7.090E-1 6.595E-1 5.807E-1 5.526E-1
Int [kHz] 2.762E+2 1.891E+2 3.409E+2 1.258E+2 5.144E+1 1.016E+2 9.905E+1 4.313E+1
Int_corr [kHz] 2.816E+2 1.920E+2 4.010E+2 1.408E+2 5.472E+1 1.085E+2 1.336E+2 6.187E+1
# samples 735 735 619 619 213 213 66 66 30 30 19 19
# cells 295

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 3741.218 - sd = 2241.152Int_corr [kHz] - Ch1 - Nuc : ave = 281.559 - sd = 191.957r = 0.789 - r^2 = 0.623
f_1 - Ch1 - Nuc : ave = 0.859 - sd = 0.179D_1 [mum^2/s] - Ch1 - Nuc : ave = 2.470 - sd = 4.369r = -0.478 - r^2 = 0.229
f_2 - Ch1 - Nuc : ave = 0.141 - sd = 0.179D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.217 - sd = 0.098r = 0.018 - r^2 = 0.000
CPM_corr [kHz] - Ch1 - Nuc : ave = 0.478 - sd = 0.255D_m [mum^2/s] - Ch1 - Nuc : ave = 1.937 - sd = 2.887r = 0.258 - r^2 = 0.066
c_corr [nM] - Ch1 - Nuc : ave = 3741.218 - sd = 2241.152ratioG_corr - Cross - Nuc : ave = 0.133 - sd = 0.123r = 0.157 - r^2 = 0.025
c_corr [nM] - Ch1 - Cyto : ave = 425.587 - sd = 734.251Int_corr [kHz] - Ch1 - Cyto : ave = 54.724 - sd = 108.469r = 0.743 - r^2 = 0.553
f_1 - Ch1 - Cyto : ave = 0.871 - sd = 0.128D_1 [mum^2/s] - Ch1 - Cyto : ave = 26.012 - sd = 24.872r = 0.053 - r^2 = 0.003
f_2 - Ch1 - Cyto : ave = 0.129 - sd = 0.128D_2 [mum^2/s] - Ch1 - Cyto : ave = 0.672 - sd = 0.428r = -0.171 - r^2 = 0.029
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.709 - sd = 0.659D_m [mum^2/s] - Ch1 - Cyto : ave = 23.931 - sd = 24.432r = -0.293 - r^2 = 0.086