Automated F(C)CS analysis of clone # ZNF183104

Created on 12/08/2014
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Gene and protein information

Clone # ZNF183104
Gene symbol: RNF113A
Description: ring finger protein 113A
Molecular weight (with FP): 65.8 kDa
Localisation: homogeneous nucleus, dim nucleoli, no speckles, no cytoplasm

Image and FCS data

Images Ch1 (EYFP construct), Ch2 (H2B-mCherry) with measurement points
ZNF183104_img_ch1.pngZNF183104_img_ch2.png


Point 1, intensity traces and correlation functions
ZNF183104_itr_1.pngZNF183104_cor_1.png

Point 2, intensity traces and correlation functions
ZNF183104_itr_2.pngZNF183104_cor_2.png

Point 3, intensity traces and correlation functions
ZNF183104_itr_3.pngZNF183104_cor_3.png

Point 4, intensity traces and correlation functions
ZNF183104_itr_4.pngZNF183104_cor_4.png

Point 5, intensity traces and correlation functions
ZNF183104_itr_5.pngZNF183104_cor_5.png

Point 6, intensity traces and correlation functions
ZNF183104_itr_6.pngZNF183104_cor_6.png

Sessions

1 20120202\U00--V00--ZNF183104
2 20120207\U00--V00--ZNF183104
3 20120221\U00--V00--ZNF183104
4 20120306\U00--V00--ZNF183104

Results table

Ch1 ave
Nuc		 Ch1 sd
Nuc		 Ch2 ave
Nuc		 Ch2 sd
Nuc		 Cross ave
Nuc		 Cross sd
Nuc		 Ch1 ave
Cyto		 Ch1 sd
Cyto		 Ch2 ave
Cyto		 Ch2 sd
Cyto		 Cross ave
Cyto		 Cross sd
Cyto
N 1.656E+2 8.286E+1 1.256E+3 5.681E+2 2.845E+1 2.233E+1 2.244E+1 3.413E+1 3.391E+2 4.270E+2 2.779E+1 2.689E+1
N_corr 1.710E+2 8.695E+1 1.679E+3 9.672E+2 3.079E+1 2.444E+1 2.036E+1 3.361E+1 4.005E+2 5.101E+2 2.774E+1 2.611E+1
c [nM] 1.030E+3 5.153E+2 5.826E+3 2.635E+3 1.463E+2 1.148E+2 1.396E+2 2.122E+2 1.573E+3 1.980E+3 1.429E+2 1.383E+2
c_corr [nM] 1.063E+3 5.408E+2 7.790E+3 4.486E+3 1.583E+2 1.256E+2 1.267E+2 2.090E+2 1.858E+3 2.366E+3 1.426E+2 1.342E+2
ratioG 1.481E-1 6.440E-2 3.829E-1 1.265E-1
ratioG_corr 1.797E-2 3.466E-2 2.509E-1 1.387E-1
f_1 9.343E-1 5.547E-2 4.581E-1 2.206E-1 9.868E-1 3.450E-2 9.258E-1 5.070E-2 4.924E-1 3.904E-1 9.763E-1 5.304E-2
tauD_1 [ms] 1.252E+0 8.062E-1 1.860E+0 2.220E-16 6.952E-1 4.924E-1 6.407E-1 1.586E+0 1.860E+0 2.220E-16 4.743E+0 5.698E+0
D_1 [mum^2/s] 1.065E+1 4.126E+0 6.926E+0 8.882E-16 2.827E+1 2.241E+1 3.185E+1 1.659E+1 6.926E+0 8.882E-16 5.765E+0 4.405E+0
alpha_1 9.854E-1 6.354E-2 9.200E-1 1.110E-16 8.580E-1 1.562E-1 1.034E+0 9.686E-2 9.200E-1 0.000E+0 1.047E+0 2.189E-1
f_2 6.569E-2 5.547E-2 5.419E-1 2.206E-1 1.321E-2 3.450E-2 7.420E-2 5.070E-2 5.076E-1 3.904E-1 2.372E-2 5.304E-2
tauD_2 [ms] 1.654E+1 7.576E+0 7.721E+1 8.915E+1 3.950E+1 1.843E+1 1.314E+1 1.209E+1 1.809E+1 1.597E+1 5.138E+1 1.282E+1
D_2 [mum^2/s] 7.478E-1 2.561E-1 4.924E-1 5.408E-1 3.974E-1 3.854E-1 1.198E+0 5.745E-1 1.693E+0 1.407E+0 2.549E-1 8.920E-2
alpha_2 1.163E+0 2.465E-1 1.218E+0 2.626E-1 1.093E+0 9.901E-2 1.036E+0 9.145E-2 1.305E+0 4.070E-1 1.103E+0 5.964E-2
tauD_m [ms] 2.361E+0 1.784E+0 4.268E+1 5.442E+1 1.229E+0 2.245E+0 1.709E+0 2.487E+0 7.534E+0 6.382E+0 6.054E+0 5.851E+0
D_m [mum^2/s] 1.004E+1 3.938E+0 3.465E+0 1.388E+0 2.802E+1 2.237E+1 2.985E+1 1.634E+1 4.391E+0 1.924E+0 5.637E+0 4.419E+0
R [1/s] 5.380E+1 3.411E+1 5.118E-1 1.144E+0
CPM [kHz] 1.443E+0 2.320E-1 3.040E-1 2.741E-1 6.728E-1 3.475E-1 2.211E-1 1.327E-1
CPM_corr [kHz] 1.457E+0 2.323E-1 3.899E-1 3.628E-1 9.404E-1 5.970E-1 1.022E+0 1.903E+0
Int [kHz] 2.581E+2 1.192E+2 1.787E+2 4.598E+1 1.180E+1 1.003E+1 5.619E+1 4.611E+1
Int_corr [kHz] 2.684E+2 1.240E+2 2.917E+2 9.281E+1 1.255E+1 1.017E+1 7.873E+1 6.641E+1
# samples 117 117 80 80 78 78 97 97 8 8 6 6
# cells 42

Histograms


c_corr [nM] - Ch1 - Nuc : ave = 1063.320 - sd = 540.791Int_corr [kHz] - Ch1 - Nuc : ave = 268.408 - sd = 124.020r = 0.941 - r^2 = 0.885
f_1 - Ch1 - Nuc : ave = 0.934 - sd = 0.055D_1 [mum^2/s] - Ch1 - Nuc : ave = 10.652 - sd = 4.126r = 0.163 - r^2 = 0.027
f_2 - Ch1 - Nuc : ave = 0.066 - sd = 0.055D_2 [mum^2/s] - Ch1 - Nuc : ave = 0.748 - sd = 0.256r = -0.281 - r^2 = 0.079
CPM_corr [kHz] - Ch1 - Nuc : ave = 1.457 - sd = 0.232D_m [mum^2/s] - Ch1 - Nuc : ave = 10.037 - sd = 3.938r = 0.374 - r^2 = 0.140
c_corr [nM] - Ch1 - Nuc : ave = 1063.320 - sd = 540.791ratioG_corr - Cross - Nuc : ave = 0.018 - sd = 0.035r = -0.227 - r^2 = 0.052
c_corr [nM] - Ch1 - Cyto : ave = 126.653 - sd = 209.007Int_corr [kHz] - Ch1 - Cyto : ave = 12.550 - sd = 10.172r = 0.579 - r^2 = 0.335
f_1 - Ch1 - Cyto : ave = 0.926 - sd = 0.051D_1 [mum^2/s] - Ch1 - Cyto : ave = 31.850 - sd = 16.594r = 0.132 - r^2 = 0.017
f_2 - Ch1 - Cyto : ave = 0.074 - sd = 0.051D_2 [mum^2/s] - Ch1 - Cyto : ave = 1.198 - sd = 0.574r = -0.339 - r^2 = 0.115
CPM_corr [kHz] - Ch1 - Cyto : ave = 0.940 - sd = 0.597D_m [mum^2/s] - Ch1 - Cyto : ave = 29.852 - sd = 16.335r = 0.397 - r^2 = 0.158